Evaluation of Acute Toxicity and Anti-Rabies Activity of Convolvulus Kilimandschari, Kalanchoe Lanceolate and Stephania Abyssinica, Traditionally Used for the Treatment of Rabies in Arsi Zone, Oromia Regi onal State, Ethiopia.
| dc.contributor.advisor | Nigatu Wondatier | |
| dc.contributor.advisor | Desta Kassu | |
| dc.contributor.advisor | Deriba Regassa | |
| dc.contributor.author | Alemu Anberber | |
| dc.date.accessioned | 2021-12-30T05:28:44Z | |
| dc.date.accessioned | 2023-11-06T08:57:10Z | |
| dc.date.available | 2021-12-30T05:28:44Z | |
| dc.date.available | 2023-11-06T08:57:10Z | |
| dc.date.issued | 2021-10 | |
| dc.description.abstract | Background; -Rabies is viral zoonotic disease progressive neurological infection which causes encephalitis in all warm-blooded animals. Dogs are the greatest reservoir host and transmit thedisease to other animals and man. It is 100% fatal after the on-set of clinical manifestation of thedisease. Plants are widely used conventionally for the treatment of rabies and contribute to thedevelopment of modern drug and treatment of this disease. Objective: -The objectives of this study was to assess the acute toxicity and anti-rabies activityeffect of C. kilimandschari, K. lanceolate and S. abyssinica, traditionally used for the treatment ofhuman rabies. Methods: - An experimental study was conducted between February, 2020 and June, 2021. In vivoacute toxicity and anti-rabies activity test was done in 78 Swiss albino mice. Extract was preparedfrom the powdered study plant using 70 % hydro-ethanol and concentrated by using rotaryevaporator. Blood samples were collected for the assessment of biochemical parameters. Brainsamples were collected for confirmatory test of rabies virus. Data was analyzed using SPSS version26 for windows software, Statistical analysis was undertaken by one-way analysis of variance(ANOVA). Results; -In this experiment, 20,15and 30gm crude extract / yield/ obtained from 100gm powdered 70%hydro ethanol extracted of C. kilimandschari, K. lanceolate and S. Abyssinia respectively.The crude extract of S. Abyssinica did exhibit sign of toxicity at dose 550mg/kg treatment groupsof mice, The LD50 of Abyssinica was 560mg/kg.C. Kilimandschari and K. lanceolate did notexhibited any sign of toxicity at all doses levels. The level of serum enzyme ALPand CHO ofmice treated with C. Kilimandschari were highly reduced. However, ALT, AST, BILD, BILT,Urea and K were increased in treated mice with K. lanceolate compared to negative control. Allplant crude extract did show significantly(p<0.05) increase in the survival period of time comparedto positive control group of mice and they have anti-rabies activity in all dose of crude extracts. Conclusion: - All plant extracts had demonstrated significant anti-rabies virus survival rate ascompared to positive control groups. However, there is a need of large scale and detail studiesshould be designed. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/29432 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | Acute toxicity, Anti-rabies activity, Stephania Abyssinica, Convolvulus Kilimandschari and kalanchoe Lanceolate. | en_US |
| dc.title | Evaluation of Acute Toxicity and Anti-Rabies Activity of Convolvulus Kilimandschari, Kalanchoe Lanceolate and Stephania Abyssinica, Traditionally Used for the Treatment of Rabies in Arsi Zone, Oromia Regi onal State, Ethiopia. | en_US |
| dc.type | Thesis | en_US |