Veterinary Obestrics and Gynaecology

Permanent URI for this collection

http://etd.aau.edu.et/handle/123456789/211

Browse

Browsing Veterinary Obestrics and Gynaecology by Subject "Antibiotics"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Cultural Characterization, Molecular Detection and In-Vitro Antibiotic Susceptibility Profile of Streptococcus equi subsp. Equi from Donkeys Suspected of Strangles in Central Ethiopia
    (Addis Ababa University, 2012-06-01) Manyahilushal Etana; Tesfaye Sisay
    Cultural characterization and molecular detection of Streptococcus equi subsp. equi from strangles suspected naturally infected donkeys was conducted in central Ethiopia from September 20 II to April, 2012 . Antibiotic susceptibility profile of the isolates was also studied. Basic bacteriology for isolation and characterization, PCR for genome detection and disc method for antibiotic susceptibility were used. A total of 87 donkeys were sampled. Out of these 21 (24.1 %) donkeys had only nasal discharge, 47 (54%) had both nasal discharge and swelling of either submandibular or retropharyngeal lymph nodes, 2 (2.3%) had parotid lymph node involvement, 12 (13.8%) had severe guttural pouch empyema and extended head and neck with strider breathing and also 5 (5 .8%) donkeys chondroid positive at postmortem. Young donkeys were affected with severe form of the disease, swelling of lymph nodes of the head region and guttural pouch empyema, than adults (p<0.05). Culturally, a total of 189 bacterial isolates belonging to II genera were obtained. These were Streptococcus spp. (42.3%), Staphylococcus spp. (18%), Neisseria spp. (1 .6%), Enterococcus spp. (10.1 %), Bacillus spp. (7.9%), COlynebaclerium spp. (4.8%), Rhodococcus spp. (2.1 %), Pasteurella spp. (5.8%), AClinomycels spp. (3.7%), Pseudomonas spp.( 2.1 %) and Proteus spp. (1.6%). Swab samples had contributed for the majority of the isolates (87.8%). The isolation rate of S. equi subsp. equi from aspirate samples was 40% being significantly (p<0.05) higher than swab samples 14%. The detection rate by PCR was 72% and 87% from swabs and aspirates samples, respectively. The molecular method was more sensitive (p<0.05) compared to cultural method . which was significantly (p<0.05) higher compared to cultural method. The in- vitro antibiotic susceptibility test of S. equi subsp. equi revealed that all isolates were found to be susceptible to ampicillin, gentamicin, chloramphenicol, norfloxacin, erythromycin, vancomycin, cephalothin, doxycycline, ceftriaxone and tetracycline; however, all of the isolates were resistant to sulphonamide, kanamycin and streptomycin. Despite its lack of sensitivity relative to PCR, cultme is of value because it definitively establishes infection. Routine use of both microbial culture and PCR analysis of samples maximize detection of S. equi subsp. equi. Aspirates, when possible, are the preferred sample to increase the sensitivity of both culture and PCR assays