Browsing by Author "Tsegaye, Aster (MSc, PhD)"

Now showing 1 - 11 of 11
  • No Thumbnail Available
    Item
    Assessment of hematological parameters among gasoline exposed workers at gas station in Mekelle City, Tigray Region, Northern Ethiopia.
    (Addis Ababa Universty, 2018-06) Teklu, Gebre; Tsegaye, Aster (MSc, PhD)
    Background: Petrol station attendants are chronically exposed to gasolineof the volatile fraction during vehicle refueling. The adverse health effects of gasoline exposure may be related to impairment of the hemopoietic system with bone marrow depression and an increased risk of developing cancer. Objective: To assese the hematological parameters among gasoline exposed workers at gas station in Mekelle City, Tigray Region, Northern Ethiopia. Method: A cross sectional study was carried on 43 individuals working in 9 petrol filling stations and 77 age and sex matched non exposed controls. Socio-demographic and exposure duration of workers were collected using structured questionnaire and observation checklist. Hematological analysis was performed using Sysmex XP -300 and peripheral blood morphology was determined. Data were entered and analyzed using SPSS version 23. Results: 28/43 (65.1%) of exposed individuals and 49/77 (63.6%) of controls were males. The average exposure time was 5.19 ± 4.38 years with an average workig hour of 11.74 ± 1.89 hours/day. The mean RBC count (1012/L), HCT (%), HGB (g/dl) and platelets count (109/l) of the exposed group were significantly lower (4.88±0.573, 43.29±3.71, 15.04±1.33 and 248.95±58.19) compared with controls (5.35±0.533, 44.95±3.10, 15.59±1.26 and 292.45±62.17) at (P<0.05) respectively. The MCH (pg) (30.48±2.06 vs 29.52±1.66) and MCHC (g/l) (34.83±0.988 vs 34.32±0.927) were significantly higher in the exposed compared with controls (P<0.05). HCT, RBC, HGB and platelets count were significantly decreased with increased year of exposure (P<0.05). The peripheral blood film examination reveals basophillic stippling and macrocytosis. Conclusion: In this study long term exposure to gasoline at gas station has deleterious effect on the RBC indices and platelet. Significant negative correlation was noted between long term exposures to gasoline and a decrease in HGB, HCT and platelet count. Thus, protective measure should be implemented by the concerned ministries with labour union to minimize exposures to gasoline fuel.
  • No Thumbnail Available
    Item
    Assessment of Patient satisfaction towards Clinical laboratory services among Strengthening Laboratory Management towards Accreditation (SLMTA) Program Implementing Hospital Laboratories under Addis Ababa City Administration, Ethiopia
    (2017-11) Tefera, Zigba; Tsegaye, Aster (MSc, PhD)
    Introduction: Patient satisfaction is a major component of a quality management system, and a significant requirement in the ISO standards. It is also an important and useful quality improvement tool for clinical laboratory, health care organizations, and business in general. Most clinical laboratories are required to assess their patients satisfaction in order to maintain their accreditations. Objectives: To assess patient satisfaction on selected laboratory services and determine associated factors among SLMTA program implementing Hospital laboratories in Addis Ababa. Methods: Hospital based cross sectional study was conducted to assess patient satisfaction on laboratory services among SLMTA program implementing hospital laboratories at Zewditu memorial, Ras Desta Damtew memorial, Yekatit 12, Terunesh-Bejieng, Gandi memorial and Minilik II hospitals in Addis Ababa. A structured questionnaire was used to interview a total of 596 laboratory services user patients by trained data collectors. Data was entered and analyzed using SPSS version 20. Patient satisfaction was determined based on mean score cutoff. Result: Out of the 596 patients, 317 (53%) were satisfied with the laboratory services given in the six hospitals under Addis Ababa City Administration. There was significant association between educational status of respondents, SLMTA program laboratory STAR grade and turnaround time with level of satisfaction of patients (P- value < 0.05). In Likert Scale, the overall mean rate of satisfaction of patients with laboratory services was 3.92 out of the 5 points. The mean rate of satisfaction for different aspects of laboratory services ranged from 3.18 to 4.34. The highest mean rate of satisfaction were given for location of laboratory and measures taken to assure privacy during specimen collection with mean rating of 4.34 and 4.32 respectively. Patient satisfaction was lowest with the adequacy of sitting arrangement in waiting area, Cleanness of Latrine and Time spent waiting to get the laboratory result. Conclusion: The overall degree of patients’ satisfaction with the clinical laboratory services was not satisfactory. Therefore, hospital administration and the laboratory department of each Hospitals should strive more to enhance patients’ satisfaction by addressing the identified gaps.
  • No Thumbnail Available
    Item
    The association of copy number variation of fcyriiib gene with the risk of enl in lepromatous leprosy patients from selected sites in Ethiopia
    (Addis Ababa Universty, 2018-01) Tsehay, Dareskedar; Tsegaye, Aster (MSc, PhD)
    Introduction: Erythema nodosum leprosum (ENL) is a Type 2 leprosy reaction which causes significant morbidity and mortality. It is characterized by systemic involvement. Despite its devastating nature, the underlying immunologic mechanisms of ENL have not been fully understood.Many researche findings have shown an association between low copy numbr of Fcgamma receptorIIIB (FcIIIB) and immune complex associated diseases. We hypothesize that the development of ENL in lepromatous leprosy patients is associated to a low copy number variation (CNV) of FcIIIB. Objective: To investigate the association of the CNV of FcRIIIBgene with the risk of ENL Methods: A case-control study was conducted on total of100 patients from selected sites in Ethiopia; ENL patients (n=50) as cases and lepromatous leprosy (LL) patients with no history of ENL (n=50) as controls from April 2017 to June 2018. Clinical information was collected using ENL International Study group form. Blood sample (2.5ml) was collected on PAXgene tube And DNA was extracted using PreAnalytix PAX gene kit, followed by a quantitative Real Time-Polymerase Chain Reaction to assess genetic association of ENL with CNV of FCIIIB gene. Clinical data was double entered in REDCap- Research Electronic Data Captureand analyzed using Graphpad prism version 7 and STATA version 11. P value less than 0.05 was considered statistically significant. Result:The relative copy number of FCIIIB was, median fold change (FC) =1.16 with 95%CI: (1.18-2.32)in cases as compared to controlsbut, the result was not statistically significant (p=0.05). There was also no statistically significant association between relative copy number and the clinical stages of ENL (p=0.07). Conclusion: This study showed that there was no statistically significant difference inthe relative copy number of FCIIIB in ENL patients as compared to controls. Further studies should be done to address the polymorphism of FCIIIB andexpression of FCIIIB on neutrophils should be measured using flow cytometry.
  • No Thumbnail Available
    Item
    Comparative study on the magnitude of intestinal parasites, Salmonella and Shigella species and Antimicrobial Susceptibility pattern among HIV infected and non-infected patients with diarrhea in selected health facilities, Dessie town, Northeast Amhara Region, Ethiopia
    (Addis Ababa Universty, 2018-06) Belay, Assefa; Tsegaye, Aster (MSc, PhD)
    Diarrhea is a common complication and manifestation of HIV/AIDS, occurring in almost 90% of HIV/AIDS infections both in adults and children in developing countries like Ethiopia. Salmonella and Shigella infections are major global public health problems causing mild to severe forms of intestinal tract infection and diarrhea; Intestinal parasites are also additional burden in developing countries. Objective: To determine the prevalence of intestinal parasites, Salmonella and Shigella Species and their antimicrobial susceptibility pattern among HIV infected and non-infected patients with diarrhea in selected health facilities, Dessie town, Northeast Amhara Regional State. Methods: Health facility based cross sectional study was conducted at Dessie Referral Hospital, Dessie Health Center and Banbua wuha Health Center from January 2018 to April 2018. A total of 354 HIV infected and non-infected diarrheic outpatients were included using convenient sampling technique. Data on socio-demographic and clinical characteristics were collected using interview that employed structured questionnaire. Freshly voided stool specimen was used for investigate intestinal parasite, culturing and antimicrobial susceptibility testing. Data was entered and analyzed using SPSS software version 22 (IBM). Results: Among 354 study participants, 112 were HIV infected and 242 were non HIV infected with diarrhea. The prevalence of Intestinal parasite and Bacterial infection among HIV infected was 26(23.2%) and 8(7.1%), respectively. Of the bacterial isolates, Salmonella accounts 6(5.4%) followed by Shigella 2(1.8%) among bacterial isolates. Moreover, the most prevalent parasite was C. parvum 9(8%), E. histolytica/dispar 8(7.1%), 4(3.6%) and 1(0.9%) co-infection of C. parvum and C. catananyesis. The bacterial isolates from diarrheal patients were 100% susceptible to Ceftriaxone 95.4% susceptible to Ciprofloxacin However, the isolates 100% resistant to Ampicillin and Amoxicillin. Conclusion: High prevalence of enteric pathogens and high resistance of isolates to commonly prescribed antibiotics were observed from HIV infected and non-infected patients. Therefore, Prevention methods should be given attention to reduce the infection rate and AST should be practiced in the health facilities to select appropriate antimicrobial agent and prevent emergence of drug resistant bacteria.
  • No Thumbnail Available
    Item
    Establishment of Reference intervals for common hematology test parameters from apparently healthy geriatrics in Asella town, southeast Ethiopia
    (Addis Abeba University, 2020-06) Hashim, Mohammed; Tsegaye, Aster (MSc, PhD); Wordofa, Moges (MSc)
    Background: Reference intervals are an important tool to identify abnormal laboratory test results. Hematology reference values are useful to interpret hematology results and make clinical decisions, but these values have not been established for geriatrics in Asella town. Objective: To establish reference intervals for common hematology test parameters from apparently healthy geriatrics in Asella town, southeast Ethiopia from January to April 2020. Methods: A community-based cross-sectional study was conducted from January to April 2020. Interviewer administered questionnaire was used to collect data of sociodemography and other characteristics from 342 eligible geriatrics. Weight, height, blood pressure, MUAC and temperature were measured and 8 milliliter blood sample was collected. Screening tests like HIV, HBsAg, HCV, syphilis, concentration method and wet mount of stool examination and urinalysis were performed. Hematological parameter was measured by Sysmex kx-21 hematology Analyzer. The data were analyzed by SPSS version 21. The non-parametric independent Mann–Whitney U test were used to compare the parameters between two gender groups. Result: The reference intervals of red blood cell, white blood cell, platelet count, hemoglobin (HGB), and hematocrit (HCT) in male Geriatrics were 3.8–5.85 × 10 /L, 3.1–9.66 × 10 9 /L, 115.8–353 × 10 9 /L, 12.4-17.76g/dL and 35.06-50.2%.The respective values for females were 3.94-5.48×10 12 /L, 3.13-8.4× 10 9 /L, 137.5-406× 10 /L, 12.516.4g/dL, and 36.09-48.2%.By comparing the two genders by non parametric Mann– Whitney U test, most of the hematological parameters showed significant differences among two genders (p-value <0.05). Conclusion: The reference intervals which were established by this study was different from the existing reference values as well as from other african countries and caucasion population. This difference could be due to difference in dietary pattern, ethnic difference as well as in the methodological used.
  • No Thumbnail Available
    Item
    Evaluation of Logistic System on Hematology commodities in Nifas Silk Lafto Sub City Public Health Centers, Addis Ababa, Ethiopia
    (Addis Abeba University, 2021-02) Nigussie, Getaye; Tsegaye, Aster (MSc, PhD); Desale, Adinew (MSc)
    Background: Laboratory logistics management information system (LLMIS) is the management of laboratory commodities, such as reagents, consumables, chemicals, equipment and other durables in a systematic and standardized way. Its purpose is to obtain and move commodities in a timely fashion to the places where they are needed at a reasonable cost with acceptable quality Objective: To assess Logistic system on Hematology commodities in Nifas Silk Lafto sub city Public health centers, Addis Ababa Methods: A facility based cross-sectional descriptive study was conducted on 10 health facilities from Jan – Feb, 2020 on the logistics management information system of hematology analyzer reagents in Nifas Silk Lafto sub city Addis Ababa. Quantitative and qualitative data collection methods were employed. The quantitative data were entered and analyzed using the Statistical Package for the Social Sciences version 20 (SPSS). Descriptive statistics computed and results presented using tables and graphs. Chi-square test was used to see the association between selected indicators with reported management supports. Results:A total of 20 pharmacy and 44 Laboratory professionals participated in the study from the ten health centers. 3(15%) and 11(55%) of the facilities had 1-5 IPLS and Lab commodity management trained pharmacy staffs respectively. A significant association observed in management enforcement on use of IFRR for reporting & resupply with calculated consumption equal in RRF and CC at all periods( p=0.032 ). From laboratory participants 17(38.6%) use all IPLS formats with regard to stock out status it was stated by 12(27.3%) of the participants. Most frequent stock out Hematology reagents were control, cell pack, diluent and detergent with 20(45.5%), 7(15.9%), 7(15.9%) and 5(11.4%) respectively. 37(84.1%) laboratory participants had got IPLS training through formal training 17(38.6%), colleague 25(56.8%) and 2(4.5%) from workshop on LMIS respectively. 27(61.4%) were agree that, developing system management will improve LMIS. Causes of successful implementation of LMIS were, 30(68.1%) staff interest, 6(13.6%) of them knowledge of the project, and team work and 2(4.5%)of them managerial support. Conclusion and recommendation: Management enforcement on use of IFRR for reporting & resupply is important for IPLS implementation. Integrated management support and supervision is recommended for proper implementation of IPLS.
  • No Thumbnail Available
    Item
    Immuno-hematological profile of individuals with podoconiosis in Yilmana Densa Woreda, West Gojjam, Ethiopia
    (Addis Ababa Universty, 2018-06) Atnaf, Aytenew; Tsegaye, Aster (MSc, PhD)
    Background: Podoconiosis is non-filarial elephantiasis, is suggested as an inflammatory disease caused by prolonged contact with irritant soil. It is prevalent in tropical Africa, including Ethiopia.. However, little is known about the immuno-hematological changes in this disease. Objective: To assess the immuno-hematological profiles of individuals with podoconiosis (Podo) in Yilmana Densa woreda, West Gojjam. Method: A case-control study was conducted from August 29, 2017-June 15, 2018 on 120 adults (53 Podo cases and 67 controls). Socio-demographic variables and associated factors for podoconiosis were collected. Stool and blood samples were analyzed for parasite identification, hematological parameters and cytokines levels. Data were entered and analyzed using SPSS 20. Results: Of the cases, 64.2% (n=53) and of the controls 67.2% (n=67) were males. Age, length of years of residence in the kebele and educational status had significant association with podoconiosis (P<0.05). Most of the cases had blood type A and B whereas controls had A and O (p>0.05). Significantly less number of cases than controls had washed their legs daily (60.4% vs 71.6%, p=0.014) and had worn shoes (88.7% versus 98.5%, p=0.023). However, less than half (45.3%) of the cases and 82.1% of the controls were wearing shoes during interview (p<0.01). Age at first leg swelling (22.6years) was less than at first shoe wearing (27.1 years). Cases had significantly lower mean WBC, GRAN, RBC, HGB, MCH, MCHC and higher mean LYMP and mixed cell population (MID) counts than controls (p<0.01). The mean CD4 count and percent, level of cytokines (IL-4, IL-6, IL-10,IL-17, IFNᵞ) of cases were not significantly different from controls (p>0.05). 37.74% of the cases and 32.84 % of the controls (p=0.576) were positive for intestinal parasites. Hookworm was the most prevalent intestinal parasite in both groups. The mean HGB level of Hook worm positive podo cases and Hook worm negative podo cases was 11.69g/dl and 12.21g/dl respectively,(F=0.061,p=0.805). Conclusion: Remarkable increase in MID and lymphocyte counts (%) but decrease in granulocyte counts (%), HGB, MCH and MCHC were seen in Podo cases. Appropriate interventions are needed to prevent multiple burdens in Podo patients and intestinal parasites in controls.
  • No Thumbnail Available
    Item
    Magnitude of Hepatitis B virus and comorbidity of intestinal parasite and Helicobacter pylori infection in School Children in Ziway, Central Ethiopia
    (Addis Abeba University, 2020-06) Girma, Roza; Tsegaye, Aster (MSc, PhD); Desta, Kassu (MSc, PhD fellow)
    Background: Hepatitis B virus (HBV) infection is globally recognized as a major risk factor for the development of liver cirrhosis and hepatocellular carcinoma in hyper endemic areas. Though some evidences shows that the association of HBV with other infections like H pylori and intestinal parasite is described in some places such information is lacking in Ethiopia. Objective: To determine magnitude of Hepatitis B Surface Antigen positivity rate and it’s comorbidity with intestinal parasites and Helicobacter pylori infections among School Children in Ziway, central Ethiopia Methods: Both retrospective and prospective cross-sectional study was conducted from October 2018 to January 2019 on samples collected from Ziway children aged 2-14 years. Socio-demographic and data related to intestinal parasites and Helicobacter pylori infection were collected from a data base which has been establi shed from a previous project. HBV surface antigen tests were performed on 348 sera using the Enzyme Linked Immunosorbent Assay (ELISA).Some socio demographic data, H.pylori status and intestinal parasites burden were taken from previous data base . Data was analyzed using SPSS version 20. Descriptive statistics was employed to determine proportions. Chi square test was employed to see any relation between HBV and demographic variables. Result was considered statistically significant at p < 0.05. Results: Out of 348 children, 179 (51.4%) were females. Most were in the age group between 59 years and accounting 49.6% of the total school children. About 31.9 % (111/348) mothers had non-formal education and 152 mothers (44.0 %) were housewives. The overall magnitude of HBsAg among school children was 3.74 % (13/348). The magnitude was higher in children aged 10-14 years (5.5%) and in males (4.2 % vs 3.4% in females), though not statistically significant. Among 324 serum samples analyzed, 235(72.5%) were positive for H. pylori antibody, 25% had protozoa and 9 % had helminthes. Neither H pylori antibody nor intestinal parasites were detected in the 13 HBsAg sero positive school children. Conclusion: Though we used a single marker for HBV infection, it calls for further studies to assess the real burden of HBV infection in the study sites. Health education should be given for the school communities and families of students to increase awareness and take preventive measures towards HBV infection.
  • No Thumbnail Available
    Item
    Phenotypic Characterization of Peripheral B cells in Mycobacterium tuberculosis infection and disease in Addis Ababa, Ethiopia
    (2018-06) Girma, Tigist; Tsegaye, Aster (MSc, PhD)
    Background: Mycobacterium tuberculosis (MTB) remains an unresolved threat resulting in great annual loss of life. The role of B cells during the protective immunity to MTB is still unclear. B cells have been described as effector cells in addition to their role as antibody producing cells during disease. Objective: to identify and characterize the frequency of peripheral B-cell subpopulations during active and latent tuberculosis. Methods: A total of 52 participants comprising 16 active untreated pulmonary TB (PTB) cases, 17 Latent Tuberculosis Infection (LTBI), and 19 Healthy Controls (HCs) were enrolled in the study. Peripheral blood samples were collected to do QuantiFERON (QFT) assay to diagnose LTBI and to isolate Peripheral Blood Mononuclear Cells (PBMC). PBMCs were stained with monoclonal antibodies. Expressions Cluster of differentiation (CD) markers was assessed on B cells subsets using multicolor flowcytometry. A difference in the frequency of B cell subsets among the groups was analyzed using the One-way Anova and Kruskal-Wallis tests was used for comparison between two groups. Data is presented as median (Interquartile range) and a P value of less than 0.05% was taken as a statistically significant difference. Results: Tissue-like memory B cells (CD19+CD27-CD21-); p = 0.0024 and class switched memory B cells (CD19+CD27+CD21+ IgM- IgD-); p = 0.0065 had significantly higher proportion in active PTB when compared to LTBI and HCs. On the contrary, resting memory B cells (CD19+CD27+CD21+); p <.0001, non-class switched memory B cells (CD19+CD27+CD21+ IgM+ IgD+); p = 0.0011, marginal zone B cells (MZCs) (CD19+CD27+CD21+IgM+IgD+CD23-); p = <0.0001 and regulatory B cells (Breg) (CD19+CD24hiCD38hiCD5+); p = 0.0043 had significantly lower proportion in active PTB group as compared to LTBI and HCs. There were no statistically significant difference observed in percentage of total B cells (CD19+); p = 02407, Naïve B cells (CD19+CD27-CD21+); p = 0.1156 and activated memory B cells (CD19+CD27+CD21-); p = 0.1134 among the three study groups. Conclusions: The findings of this study showed that B cells markers distinguish active untreated TB cases from LTBI, that indicate B cells phenotype is promising for clinical application as potential biomarker for TB disease and LTBI identification. A large scale longitudinal study is required to confirm and translate this finding into clinically applicable tests.
  • No Thumbnail Available
    Item
    Seroprevalence of HBV, HIV and Associated Factors among Pregnant Women Attending Selected Health Centers at Yeka Sub City, Addis Ababa, Ethiopia
    (Addis Ababa University, 2018-06) Getaneh, Yibeltal; Tsegaye, Aster (MSc, PhD)
    Background: Hepatitis virus causes highly contagious viral infection, which kills millions globally than any other diseases. Among those hepatic viruses, Hepatitis B virus plays pivotal role in causing hepatic failure. Morbidity and mortality increases when it combines with Human immunodeficiency virus which negatively impacts the natural history of Hepatitis B virus, increasing the risks for cirrhosis or hepatocellular carcinoma. Infection with Hepatitis B virus and Human immunodeficiency virus leads to increased morbidity and mortality as compared to hepatitis B virus infections and independent Human immunodeficiency virus Objective: To assess seroprevalence and associated factors of HBV, HIV infections among pregnant women attending ANC at selected Health Centers of Yeka Sub City, Addis Ababa, Ethiopia Methods: A health facility based cross sectional study design was conducted among 400 pregnant women attending four randomly selected Health centers of Yeka Sub City, Addis Ababa, Ethiopia between February and March 2018. Convenient sampling technique was used to enroll the pregnant women. A pretested structured questionnaire was used to collect socio-demographic characteristics and possible associated factors. HBsAg was determined using rapid screening test. Positive samples were confirmed by enzyme linked immunosorbent assay. In addition to this, antibodies to HIV-1/2 were tested based on the national testing algorithm of Ethiopia. Descriptive statistics and logistic regression analysis were done and a P value less than 0.05 was considered statistically significant. Data was analyzed using SPSS version 22. Result: A total of 400 pregnant women, of whom 4.8% of them were unmarried, enrolled in this study. Seroprevalence of 2.5% (10/400) and 0.5% (2/400) were found for HBsAg and HIV, respectively with co-infection rate of 0.25%. None of the socio-demographic and potential risk factors showed significant association, except history of surgery which showed significant association with HBV infection with an AOR=5.56, 95% CI, 1.14-27.27. Conclusion: the prevalence of current study implicates a strong need to work on the prevention of HBV and HIV transmission to the community. All pregnant women need to access screening for HBV, and HIV in their antenatal care to prevent HBV and HIV transmission.
  • No Thumbnail Available
    Item
    Stability of Complete Blood Count and 3-part White Cell Differential Parameters with Storage Time and Temperature Variation using Cell DYN 1800 Automated Hematology Analyzer
    (Addis Abeba University, 2011-07) Tesema, Desalegn; Tsegaye, Aster (MSc, PhD)
    Background: Complete blood count (CBC) and differential white cell counts are the most commonly ordered tests in clinical practice. The standard operation procedures in the ART laboratories, which are prepared based on the instrument instruction manual of the company, recommend 8 hours storage at room temperature. However, there is no clear information as to how to handle delayed specimens in situations where delayance is unavoidable. Objective: To evaluate whole blood stability with time, storage temperature and transportation conditions using Cell-Dyn 1800, a 3-part diff hematological analyzer. Methods: A total of 45 adult participants, 19 from ART clinic of Tikur Anbessa Specialized Hospital and 26 apparently healthy Medical Laboratory Science students of Addis Ababa University were included in the study using convenient sampling methods. EDTA whole blood samples were analyzed at baseline before and after aliquoting. Each of six aliquots, for room temperature and 2-8 O C storage, one each for transporting with and without an ice box were used. After the baseline analysis of the direct EDTA tube and an aliquot, all specimens were analyzed at 8, 24, 32, 56 and 72 hours of storage. Same EDTA tube was used each time after refrigeration. Transported samples were analyzed within 8 hours and compared with baseline values. Cell-Dyn 1800 automated hematology analyzer was used following the manufacturers instruction. Results: Among the CBC parameters, WBC, RBC, Hgb, MCH were stable for 72 hours post sample collection regardless of storage temperature. These values were also unaffected during transportation. MCHC was relatively less stable. PLT counts remained stable in the direct EDTA tube samples stored at 2-8 O C for 72 hours while aliquoted samples were less stable, showing a declining trend with time. The MCV and RDW, parameters affected by change in the red cell size showed an increasing trend with time where differences were statistically significant (P<0.05) after 48 hours of storage. WBC Differential data was the least stable of all, even after 8 hours of storage, where granulocytes showed a declining trend Conclusion: The various CBC parameters showed different levels of stability at different specimen storage and handling conditions. Each parameter should be treated case by case in conditions where delayance is unavoidable.