Studies on Salivary Gland Hyperplasia Virus (SGHV) Transmission In Glossina Pallidipes Colony Using Conventional and Molecular Techniques
| dc.contributor.advisor | Tekie, Habte (PhD) | |
| dc.contributor.author | Kiflom, Musie | |
| dc.date.accessioned | 2018-07-06T13:40:48Z | |
| dc.date.accessioned | 2023-11-09T04:21:49Z | |
| dc.date.available | 2018-07-06T13:40:48Z | |
| dc.date.available | 2023-11-09T04:21:49Z | |
| dc.date.issued | 2007-07 | |
| dc.description.abstract | In Ethiopia five species of tsetse flies were recorded from different parts of the country. Glossina pallidipes is one of the five species which cause a fatal disease to human and animal. Sterile Insect Technique (SIT) as a component for tsetse fly control and eradication programme is one of the successful techniques in some insect pest eradication programmes. However, the presence of salivary gland hyperplasia virus affects the application of SIT technique in tsetsecontrol as it limits the mass rearing activity. It is widely accepted that tsetse plays host to salivary gland hyperplasia virus. This study mainly focused on virus free line establishment and the antiviral drug treatment using more than 1800 G.pallidipes Tororo and 3072 G. pallidipes Arbaminch strains. Prior to the large scale experiments, two preliminary surveys were done on virus free line establishment and antiviral drug treatments. All experiments were analyzed using PCR, dissection and QPCR methods. The preliminary survey on the investigation of the virus free line by dissection showed that 4.45% male flies and 3.09% of female flies were positive for salivary gland hyperplasia, whereas in PCR analysis 95% of the flies were positive for SGHV. In the virus free line establishment a total of 920 female flies were taken by randomly and 13.04% were selected by PCR as positive or negative flies for salivary gland hyperplasia and two out of 120 female flies died. Among 120 flies, 26.7% male and 15% female including the dead flies were negative and 35.8% female and 22.5% male flies were positive by PCR. All screened flies were grouped into four groups as Virus free line I, II, III and IV. In the preliminary survey of the antiviral drug, female flies treated with valacyclovir treated flies produced more pupae than acyclovir treated flies. In the main experiment of the antiviral drugs valacyclovir treated flies showed no significant difference P>.072. But, acyclovir and control showed significant difference P<.000 and P<.005 at P=0.05, respectively. Key words: Glossina pallidipes, SIT, Salivary gland hyperplasia virus, virus free line, Trypanosomosis | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/7117 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Glossina Pallidipes | en_US |
| dc.subject | Salivary Gland Hyperplasia Virus | en_US |
| dc.subject | Virus Free Line | en_US |
| dc.subject | Trypanosomosis | en_US |
| dc.title | Studies on Salivary Gland Hyperplasia Virus (SGHV) Transmission In Glossina Pallidipes Colony Using Conventional and Molecular Techniques | en_US |
| dc.type | Thesis | en_US |