Profiling and Antimicrobial Susceptibility Pattern of Non-Fermentative Gram- Negative Bacilli Isolated from Patients Referred to Arsho Medical Laboratory Addis Ababa, Ethiopia.
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Date
2021-09
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Addis Abeba University
Abstract
Background: Non-Fermenting Gram-Negative Bacilli are defined as strictly aerobic and nonspore
forming
group
of
bacteria.
They
are
intrinsically
resistant
to
many
antibiotics.
Globally
the
burden
of multidrug resistance in Gram-negative bacilli represents a daily issue for the
management of antimicrobial therapy in patients.
Objective: The aim of this study was profiling and antimicrobial susceptible pattern of nonfermentative
Gram- negative bacilli isolated from patients referred to Arsho Advanced Medical
laboratory Addis Ababa, Ethiopia.
Methods: A cross sectional study were conducted in Arsho advanced medical laboratory Addis
Ababa, Ethiopia from January 2020 to August 2021 on 705 clinical samples. From this, isolates
were detected from different clinical samples such as urine, body fluid, blood, CSF and other
samples which was collected from patients. All the samples received in the microbiology
laboratory were inoculated on blood agar, MacConkey agar and chocolate agar and incubated at
37°C for 48hr. Non fermentative Gram-negative bacilli was isolated and identified from clinical
specimens by standard procedure and antibiotic sensitivity test was performed by VITEC 2.
Gram reaction of the organisms was determined by Gram stain.
Results: From the total of 705 various specimens, 137 (34.5%) were culture positive for Nonfermentative
Gram-negative bacteria, Among the isolated bacteria, the highest number was
Acinetobacter baumannii 26/137(3.7%). While Pseudomonas aeruginosa accounts 20/137 (2.8)
followed by Proteus mirabilis 16/137 (2.3%). Among NFGNB P. aeruginosa (100%), and A.
baumanii (100%) were multi drug resistance.
Conclusion: Antimicrobial resistance and multi drug resistance was performed during the study
period was high, it is due to ordering antibiotic without knowing the right pathogen and also
unlimited use of antibiotics.
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Keywords
Non-fermentative, Gram- negative bacilli, antimicrobial susceptible, antimicrobial resistantAddisAbaba,Ethiopia.