Studies on Optimization of Amylase Extraction Using Fabricated Extractor Column for Fiber Opening and Characterization of Enzyme
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Date
2018-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
AAU
Abstract
Amylases are important hydrolase enzymes which have been widely used since many decades. αAmylase
is
in
maximum
demand
due
to
its
wide
range
of
applications
and
plays
a
pivotal
role
such
as
leather making process. Due to absence of cheap and effective extraction method, it mostly
leads to loss of enzyme activity as well as waste of crude enzyme extract.
This present work is concerned with characterization and studies to maximize and optimize the
extraction process using fabricated extractor column from bacterial culture under solid state
fermentation (SSF) using agro industrial residue for fiber opening. Various process parameters
are optimized for maximum extraction of enzyme. The finding of the study suggests that, the
optimum condition for maximum amylase extraction with medium sized wheat bran at pH of 7.1
and temperature of 30
0
C yields 1332.31 U/gds and 2374.51 U/gds in solid state fermentation
media respectively. In continuous column extraction process with optimum buffer flowing at rate
of 100ml/min and optimum bed volume of 200gm provides a maximum activity of 1081.8 U/gds.
Comparing batch with column system of extraction, the combined activity shows 114.89 and 551.3
U/gds respectively. Moreover, by comparing the conventional with experimental method of
extraction, it shows a combined activity of 347.7 and 450.5 U/gds respectively. Hence the total
yield of column extraction process shows 75% higher as compared with batch system and
conventional method of enzyme extraction process. In order to check the benefits of α-amylase, the
crude enzyme extract is partially purified before applying it on leather. In an attempt to reduce
the pollution load from pre tanning operation, a concentrated amylase is applied on goat skin.
Hence, studying the effect of fiber opening between enzymatic and chemical process by various
tests were carried out. The finding revealed that the extracted enzyme releases 7.3 mg/gm of
proteoglycans after enzyme treatment. The effect of fiber splitting on the strength properties
related morphological changes of the crust leather samples were thoroughly investigated. SEM
analysis was also carried out. According to the result an economical enzyme produced in this
study shows a significant release of inter fibrillary materials. The strength property of crust leather
shows tensile strength of 216.4 Kg/cm
3
, tear strength of 42.76 Kg/cm and 62.17% elongation at
break.
Description
Keywords
α-amylase, bacillus subtillis, column extraction system, rust leather, enzyme extraction optimization, proteoglycan release, solid state fermentation