Comparison of Cerebrospinal and Pleural Fluid Analysis using Manual and Automated Hematology Analyzer at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia.
| dc.contributor.advisor | Tsegaye Aster | |
| dc.contributor.advisor | Alemu Rahe | |
| dc.contributor.advisor | Mamushet Yared | |
| dc.contributor.advisor | Kebede Henok | |
| dc.contributor.advisor | Tamire Tewodros | |
| dc.contributor.author | Aschenik Mulualem | |
| dc.date.accessioned | 2021-12-12T09:31:31Z | |
| dc.date.accessioned | 2023-11-06T08:57:00Z | |
| dc.date.available | 2021-12-12T09:31:31Z | |
| dc.date.available | 2023-11-06T08:57:00Z | |
| dc.date.issued | 2021-08 | |
| dc.description.abstract | Background: Analysis of body fluids provides important information for assessing various medical conditions. Bothe manual and automated hematology analyzers are used for cellcounting. However, no published study is available to validate the diagnostic performance of theSysmex 4000-i (Sysmex, Japan) system for the analysis of CSF and Pleural fluids. Objective: To compare the manual counts of cerebrospinal fluids (CSF) and pleural fluids (PF)with the Sysmex XT-4000i automated hematology analyzers. Methods: In this cross-sectional study, 240 body fluid samples were analyzed using the Sysmex4000-i system in the body fluid mode and light microscopy at Tikur Anbessa SpecializedHospital. Body fluids included were pleural and cerebrospinal fluid (CSF). The results of themanual and automated counts were compared using the Pearson correlation test. Results: A comparison between the Sysmex 4000-i system and manual counting demonstratedgreat correlations with regard to pleural fluid WBC, Polymorph nuclear cells and Mono nuclearcell counts where r =0.82, 0.96, 0.95, respectively in both methods of the counts. Cell count fromCSF also showed good correlations for both manual and automated counts of WBC, PMN % andMN% where r=0.83, 0.94, and 0.92, respectively. The Sysmex 4000-i system also demonstratedgood performance for differential cell counting (r =0.9028). CSF particularly showed a goodcorrelation. Conclusions: The use of the Sysmex 4000-i system for cell counting and differential analysis ofbody fluid samples might be an effective and automated alternative to chamber counting inlaboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/29209 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | CSF, Pleural fluid, Manual count, automated count. | en_US |
| dc.title | Comparison of Cerebrospinal and Pleural Fluid Analysis using Manual and Automated Hematology Analyzer at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. | en_US |
| dc.type | Thesis | en_US |