Comparison of Cerebrospinal and Pleural fluid analysis using manual and automated hematology analyzer at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia.
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Date
2021-08
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Addis Abeba University
Abstract
Background: Analysis of body fluids provides important information for assessing various
medical conditions. Bothe manual and automated hematology analyzers are used for cell
counting. However, no published study is available to validate the diagnostic performance of the
Sysmex 4000-i (Sysmex, Japan) system for the analysis of CSF and Pleural fluids.
Objective: To compare the manual counts of cerebrospinal fluids (CSF) and pleural fluids (PF)
with the Sysmex XT-4000i automated hematology analyzers.
Methods: In this cross-sectional study, 240 body fluid samples were analyzed using the Sysmex
4000-i system in the body fluid mode and light microscopy at Tikur Anbessa Specialized
Hospital. Body fluids included were pleural and cerebrospinal fluid (CSF). The results of the
manual and automated counts were compared using the Pearson correlation test.
Results: A comparison between the Sysmex 4000-i system and manual counting demonstrated
great correlations with regard to pleural fluid WBC, Polymorph nuclear cells and Mono nuclear
cell counts where r =0.82, 0.96, 0.95, respectively in both methods of the counts. Cell count from
CSF also showed good correlations for both manual and automated counts of WBC, PMN % and
MN% where r=0.83, 0.94, and 0.92, respectively. The Sysmex 4000-i system also demonstrated
good performance for differential cell counting (r =0.9028). CSF particularly showed a good
correlation.
Conclusions: The use of the Sysmex 4000-i system for cell counting and differential analysis of
body fluid samples might be an effective and automated alternative to chamber counting in
laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.
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Keywords
CSF, Pleural fluid, Manual count, automated count.