Establishment of Reference Intervals for Selected Clinical Chemistry Parameters among Apparently Healthy Adults in Addis Ababa, Ethiopia
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Date
2020-06
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Addis Abeba University
Abstract
Background: Reference interval values are used as medical decision-making tools that are given
by clinical laboratory to assist health professionals, medical researchers for clinical trials and for
physician to diagnosis, treatment, and follow-up of patients..
Objective: This study aimed to establish reference interval for selected clinical chemistry
parameters among apparently healthy adults living in Addis Ababa, Ethiopia.
Method: community based cross sectional study was conducted from March to October 2019
recruiting a total of 448 participants aged from 18-60 years. Medical history, physical
examination, anthropometric and socio-demographic data were collected using structured
questionnaire. Serum samples were analyzed for selected clinical chemistry parameters using
Cobas 6000. Reference intervals were established by calculating 2.5
th
-97.5
percentile with
90%Cl after excluding outliers using tukey method, Kolmogorov-Sminorv test. The difference
between groups was computed using Mann-Whitney test by using SPSS version 23. P-value of
<0.05 was taken as statistically significant.
Result: In adults the reference interval of males and females were ALT 1.60-16.89U/l against
females 0.47-9.87U/l, AST 8.11-22.50 vs.6.95-18.05 U/l, ALP 44.0-181.65U/l; Albumin 4.405.88U/l
vs.4.03-5.25U/l, T.protein 6.76-9.57g/l vs. 6.45-8.21g/l, T.bilirubin 0.26-1.15 vs.0.150.64mg/dl,
D. bilirubin 0.08-0.46mg/dl vs. 0.061-0.25mg/dl, Glucose 62.93-99.39mg/dl
vs.59.14-90.72mg/dl, Urea 13.21-33.51mg/dl vs.7.30-12.00mg/dl, Creatinine 0.56-1.02mg/dl
vs.0.40-0.80mg/dl and Uric acid 3.41-7.40mg/dl, vs.1.88-5.60mg/dl, respectively. Statistically
significant differences were observed between males and females except Albumin and Total
proteins.
Conclusion: the reference interval for selected clinical chemistry parameters of adults was lower
than the values given by manufacturers and Africans studies. Hence, it is more useful using
locally established reference interval than derived from other countries.
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Keywords
Reference Interval, clinical chemistry