Prevalence and Distribution of fungal species in sputum collected from pulmonary tuberculosis suspected Patients at Saint Peter’s Specialized Hospital, Addis Ababa, Ethiopia.
| dc.contributor.advisor | Bitew, Adane(MSc, PhD). | |
| dc.contributor.author | Bati, Solomon | |
| dc.date.accessioned | 2020-01-30T18:47:22Z | |
| dc.date.accessioned | 2023-11-06T08:56:29Z | |
| dc.date.available | 2020-01-30T18:47:22Z | |
| dc.date.available | 2023-11-06T08:56:29Z | |
| dc.date.issued | 2019-10 | |
| dc.description | This is to certify that the thesis prepared by SOLOMON BATI, entitled: “Prevalence and Distribution of fungal species in sputum collected from pulmonary tuberculosis suspected Patients at Saint Peter’s Specialized Hospital, Addis Ababa, Ethiopia” and submitted in partial fulfillment of the requirements for Master of Science degree in Clinical Laboratory Sciences (Diagnostic and Public health microbiology) complies with the regulations of the University and meets the accepted standards with respect to originality and quality. | en_US |
| dc.description.abstract | Background: Pulmonary mycosis is a systemic fungal infection that occurs when fungi cause’s disease of the lungs. Fungal pulmonary infection has been emerging recently due to widely used broad-spectrum antibiotics and steroids, immune deficiency or suppression in tuberculosis, patients are easily vulnerable to opportunistic fungal infections. Objective: To determine the distribution of pulmonary fungal isolates among pulmonary tuberculosis presumptive patients in the sputum sample by conventional culture. Methods: A cross sectional study was conducted at Saint Peters specialized hospital on 636 sputum samples from presumptive tuberculosis patients. Early morning sputum samples were collected aseptically and Presence of pulmonary Tuberculosis was screened by Xpert MTBRif/ assay. All sputum samples were cultured using Sabrouad dextrose agar and Brain heart infusion agar medium incubated at 250C and 370C for 4 weeks respectively for each sputum samples. Identification was performed by noting; the growth form and rate of growth, surface and reversed coloration. Yeasts were identified by conventional biochemical tests and assimilation characteristics. Chromo agar medium used for Candida species and urease used for Cryptococcus neoformance and the rest were non-yeast species. Data analysis was carried out using SPSS version 23 software. Results: The distribution of pulmonary fungal isolates among pulmonary tuberculosis presumptive patients was at 75.9% of the infected patients, male patients accounted for 51.4%) %, whereas female patients accounted for 48.6%.Yeast isolates (82%) and mold isolates were (18%). Among 163 tuberculosis (TB) cases 127(77.9%) were co-infected with pulmonary fungal pathogen. Conclusion: High distribution of fungal isolation (75.9%) was obtained in patients with respiratory symptoms. We recommend for policy makers to conduct further studies and consider the need of fungal screening in these patients. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/20536 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Respiratory fungal infection, presumptive TB patients, Yeast species, Filamentous fungi, Co-infection | en_US |
| dc.title | Prevalence and Distribution of fungal species in sputum collected from pulmonary tuberculosis suspected Patients at Saint Peter’s Specialized Hospital, Addis Ababa, Ethiopia. | en_US |
| dc.type | Thesis | en_US |