Prevalence and Distribution of fungal species in sputum collected from pulmonary tuberculosis suspected Patients at Saint Peter’s Specialized Hospital, Addis Ababa, Ethiopia.
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Date
2019-10
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Addis Ababa University
Abstract
Background: Pulmonary mycosis is a systemic fungal infection that occurs when fungi cause’s
disease of the lungs. Fungal pulmonary infection has been emerging recently due to widely used
broad-spectrum antibiotics and steroids, immune deficiency or suppression in tuberculosis,
patients are easily vulnerable to opportunistic fungal infections.
Objective: To determine the distribution of pulmonary fungal isolates among pulmonary
tuberculosis presumptive patients in the sputum sample by conventional culture.
Methods: A cross sectional study was conducted at Saint Peters specialized hospital on 636
sputum samples from presumptive tuberculosis patients. Early morning sputum samples were
collected aseptically and Presence of pulmonary Tuberculosis was screened by Xpert MTBRif/
assay. All sputum samples were cultured using Sabrouad dextrose agar and Brain heart
infusion agar medium incubated at 250C and 370C for 4 weeks respectively for each sputum
samples. Identification was performed by noting; the growth form and rate of growth, surface
and reversed coloration. Yeasts were identified by conventional biochemical tests and
assimilation characteristics. Chromo agar medium used for Candida species and urease used for
Cryptococcus neoformance and the rest were non-yeast species. Data analysis was carried out
using SPSS version 23 software.
Results: The distribution of pulmonary fungal isolates among pulmonary tuberculosis
presumptive patients was at 75.9% of the infected patients, male patients accounted for 51.4%)
%, whereas female patients accounted for 48.6%.Yeast isolates (82%) and mold isolates were
(18%). Among 163 tuberculosis (TB) cases 127(77.9%) were co-infected with pulmonary fungal
pathogen.
Conclusion: High distribution of fungal isolation (75.9%) was obtained in patients with
respiratory symptoms. We recommend for policy makers to conduct further studies and consider
the need of fungal screening in these patients.
Description
This is to certify that the thesis prepared by SOLOMON BATI, entitled:
“Prevalence and Distribution of fungal species in sputum collected from
pulmonary tuberculosis suspected Patients at Saint Peter’s Specialized
Hospital, Addis Ababa, Ethiopia” and submitted in partial fulfillment of the
requirements for Master of Science degree in Clinical Laboratory Sciences
(Diagnostic and Public health microbiology) complies with the regulations of the
University and meets the accepted standards with respect to originality and quality.
Keywords
Respiratory fungal infection, presumptive TB patients, Yeast species, Filamentous fungi, Co-infection