Prevalence and Antimicrobial Resistance Profile of E. coli O157:H7 Isolated from Swab of Ruminant Carcass from Municipal Abattoir and Small Butcher Shops in Akaki Kality Sub-City, Addis Ababa, Central Ethiopia
| dc.contributor.advisor | Getachew Tadessse | |
| dc.contributor.advisor | Liyuwork Asifaw | |
| dc.contributor.author | Tirumengist Bayile | |
| dc.date.accessioned | 2024-11-25T05:48:29Z | |
| dc.date.available | 2024-11-25T05:48:29Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Ethiopian traditional diets frequently include raw meat and its products, which can include the high-risk pathogen Escherichia coli O157:H7. Most of the time, E. coli O157:H7 causes antibiotic resistance in both humans and animals. Therefore, in the Akaki Kality sub-city , the investigation was carried out to ascertain the prevalence and antimicrobial resistance profile of E. coli O157: H7 isolated from ruminant carcass swabs from abattoir and butcher shops. 408 samples were taken from the randomly chosen swabs of the floor, knife, and carcass from the butcher and abattoir that were investigated; of them, 11.76% (48/408) tested positive for E. coli O157:H7. Regarding the sample source, out of all the samples analyzed, the butcher shop had the highest overall prevalence of E.coli O157:H7 contamination (16.67% (30/180), followed by the abattoir sample (7.89% (18/228). Significant variations in prevalence were noted between sample sources (p<0.05). E. coli O157:H7 was highly prevalent in bovine, caprine and ovine (13.3% (24/180), 11.67% (14/120, and 9.26% (10/108), respectively, when it came to species. Based on the type of sample used in this investigation, the highest prevalence rate of E.coli O157:H7 was found in carcasses (14.5%) (29/200), followed by floors (12.1%) (12/108), and knives (7%/7/100). Using ten antimicrobial discs that were on hand, the antimicrobial susceptibility testing of 48 E.coli O157:H7 isolates showed that the isolates were susceptible to 100% of trimethoprim, 97.32% of oxytetracycline, 89.58% of gentamycin, and 83.33% of kanamycin. Tetracycline (87.5%), ampicillin (85.42%), and streptomycin (93.75%) had the highest rates of antimicrobial resistance. The high rate of antibiotic resistance and the general high prevalence of E. coli O157:H7 thus suggest that there may be a risk to patient safety and medication efficacy. Based on the findings of this study, government officials, project managers, and veterinarians should collaborate to establish more stringent oversight of antibiotic usage in both animal and human population. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/3674 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Abeba University | |
| dc.subject | Abattoir | |
| dc.subject | Akaki kality | |
| dc.subject | Antimicrobial Resistance | |
| dc.subject | E.coli O157:H7 | |
| dc.title | Prevalence and Antimicrobial Resistance Profile of E. coli O157:H7 Isolated from Swab of Ruminant Carcass from Municipal Abattoir and Small Butcher Shops in Akaki Kality Sub-City, Addis Ababa, Central Ethiopia | |
| dc.type | Thesis |