Identifications of Intestinal Parasites, Salmonella and Shigella Species and Knowledge, Attitude and Practice of Fruit Juice Makers in Selected Sub Cities of Addis Ababa, Ethiopia.
| dc.contributor.advisor | Desta Kassu | |
| dc.contributor.advisor | Alemayehu Haile | |
| dc.contributor.author | Tsegaye Henok | |
| dc.date.accessioned | 2021-11-16T07:52:17Z | |
| dc.date.accessioned | 2023-11-06T08:56:59Z | |
| dc.date.available | 2021-11-16T07:52:17Z | |
| dc.date.available | 2023-11-06T08:56:59Z | |
| dc.date.issued | 2021-11 | |
| dc.description.abstract | Background: Unpasteurized fruit juices known to cause food poisoning from harmful germssuch as Salmonella, Shigella and gastrointestinal parasites. Fruits are susceptible to differentmicrobes if didn’t prepared practice good hygiene in overall process. Due to that Food bornediseases are the main public health concerns in the world, especially in developing countries. Objective: To identify Intestinal Parasites, Salmonella and Shigella species from consumed localfruit juices sample and juice makers and assessing their Knowledge, Attitude and Practice offruit juice makers. Methods: A Cross-Sectional study was conducted at selected sub cities of Addis Ababa,Ethiopia. Knowledge, Attitude, Practice assessment questionnaire was taken to evaluate the factors to microbial quality and safety of local fruit juices. Different varieties of fruit juices andstool samples from juice makers was collected and examined for microbiological tests fromFebruary 2021 to June 2021.Juice samples were pre-enriched in Buffer Phosphate Waterfollowed by selecting enrichment for isolation, culture, biochemical tests, Becton Dickinsonphoenix analyzers, serotyping were used for confirmation of those pathogens. Antimicrobialsusceptibility tests were also carried out using 10 antibiotic discs on each isolate by using KirbyBauerdiscdiffusio method.ThedatawasenteredintoEpi infversion3.5.4 andanalyzedusingSPSS version23. A p value≤ 0.05 at95% CIwasconsideredasstatisticallysignificant. Results: A Total of 100 fruit juice sample, 100 fruit juice makers fecal samples, 120 KAPassessments were enrolled in the study. The majority study participants were female 108(90%).About 27 (27%) and 17(17%) of juice makers and fruit juice samples were positive for differentintestinal parasites with the most abundant parasite of A. lumbricoides 7(7%) and E.histolytica5(5%) from juice samples and juice makers respectively. Stool and juice cultures revealed 6%and 5% of Salmonella isolates. While Shigella species was not isolated from both sample types.The participants level of knowledge and attitude score were 95.8% and 63.1% and the practice level score were 39.5%. S.Dublin were the only serotype found in both fruit juices and stoolspecimen. All isolates of Salmonella were sensitive to Ciprofloxacin, Chloramphenicol,Sulfamethoxazole trimethoprim, Nalidixic acid, Ceftriaxone and only Gentamycin andTetracycline shows the highest intermediate. But resistant to Erythromycin, Ampicillin,Amoxicillin Clavunilic acid. Conclusion: The results clearly illustrated the fresh juices preparations didn’t meet public healthstandards. They know their work but poor practice indicated. Such foods are responsible tocauses food borne diseases specially carrier to many kinds of intestinal parasites andenteropathogenic bacterias. Therefore, policy makers and implementers must adopt strictmeasures and supervision to the vendors about food safety and hygienic practices to update theguidelines and standards for fruit products preparations. Certain antibiotics for Salmonella fromfecal and non-fecal source gives exceptional result for further study since multiple drugresistance of many antibiotics still differentiated that affect drug viability to Salmonella species. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/28696 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | Fruit juice, Contaminations, Salmonella species, Shigella species, Antimicrobial resistance, Knowledge, Attitude, Practice | en_US |
| dc.title | Identifications of Intestinal Parasites, Salmonella and Shigella Species and Knowledge, Attitude and Practice of Fruit Juice Makers in Selected Sub Cities of Addis Ababa, Ethiopia. | en_US |
| dc.type | Thesis | en_US |