ANTIMICROBIAL AND BACTERIA CULTIVATIVE VALUE OF MORINGA OLIFERA LEAF EXTRACT IN SELECTED ANIMAL PATHOGENS
| dc.contributor.advisor | Dr. Biruk Tesfaye, Dr. Tesfaye Sisay | |
| dc.contributor.advisor | Dr.BedasoMamo | |
| dc.contributor.author | ADDISU, DEMEKE | |
| dc.date.accessioned | 2020-06-18T08:00:09Z | |
| dc.date.accessioned | 2023-11-08T11:38:10Z | |
| dc.date.available | 2020-06-18T08:00:09Z | |
| dc.date.available | 2023-11-08T11:38:10Z | |
| dc.date.issued | 2014-06 | |
| dc.description.abstract | The research was conducted to evaluate the antibacterial effect of various forms of the leaf extracts (Moringa oleifera) on the growth of four selected gram-positive and negative bacteria and to evaluate the bacterial cultivated value of the extract.The antibacterial activity of leaf extracts of Moringa oleifera Lam., was determined in vitro, using disc diffusion and minimum inhibitory concentration (MIC) determined against selected animal pathogenic bacteria. M. oleiferaleave extract has been found to possess antimicrobial properties. In this work, four extraction solvents were used, i.e. aqueous extraction, fresh leave juice, ethanol and methanol. The methanol extract method of fresh and dried leaves displayed potential broad spectrum activity against all the tested organisms whereas the fresh leave juice extract which didn’t have any inhibitory effect were evaluated for its bacterial cultivated value and it was obtained that the power form of this extract was optimal environment for the growth of both gram positive and gram negative selected bacteria. The antimicrobial assay showed that the zones of inhibition produced using disc diffusion method ranged 3mm to 22mm for the four extraction methods with the highest value of 22mm obtained with ethanol extraction. The zones of inhibition for fresh ethanol extract was 4 to 22 mm, methanol extract from fresh leaf was 8 to 20 mm, and hot and cold water extract of fresh and dried leaves was 8 to 9,8 to 10,3 to 4 and 3 to 5 mm respectively. The MIC values were conducted for the different form of extracts against the tested bacterial pathogens and the highest concentration was recorded 25mg/ml for S.auerus while it was 30 mg/ml for S.fecalis. The bacterial cultivated value of M.oleifera powder extract of fresh leaf juice was determined by cultivating the four tested pathogens on the culture medium which was prepared from the plant extract with the proper concentration of agar powder in sterile distilled water and it was revealed that all the pathogens were successfully grown on it. The consequences of this investigation suggest that the extracts and juice of M.oleifera Lam. can be used to discover antibacterial agent for developing new pharmaceuticals to control animal pathogenic bacteria responsible for severe illness. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/21718 | |
| dc.language.iso | en | en_US |
| dc.subject | Antimicrobial | en_US |
| dc.subject | leaf | en_US |
| dc.subject | Moringa oleifera | en_US |
| dc.subject | Pathogenic bacteria | en_US |
| dc.title | ANTIMICROBIAL AND BACTERIA CULTIVATIVE VALUE OF MORINGA OLIFERA LEAF EXTRACT IN SELECTED ANIMAL PATHOGENS | en_US |
| dc.type | Thesis | en_US |