ANTIMICROBIAL AND BACTERIA CULTIVATIVE VALUE OF MORINGA OLIFERA LEAF EXTRACT IN SELECTED ANIMAL PATHOGENS
No Thumbnail Available
Date
2014-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The research was conducted to evaluate the antibacterial effect of various forms of the
leaf extracts (Moringa oleifera) on the growth of four selected gram-positive and
negative bacteria and to evaluate the bacterial cultivated value of the extract.The
antibacterial activity of leaf extracts of Moringa oleifera Lam., was determined in vitro,
using disc diffusion and minimum inhibitory concentration (MIC) determined against
selected animal pathogenic bacteria. M. oleiferaleave extract has been found to possess
antimicrobial properties. In this work, four extraction solvents were used, i.e. aqueous
extraction, fresh leave juice, ethanol and methanol. The methanol extract method of
fresh and dried leaves displayed potential broad spectrum activity against all the tested
organisms whereas the fresh leave juice extract which didn’t have any inhibitory effect
were evaluated for its bacterial cultivated value and it was obtained that the power form
of this extract was optimal environment for the growth of both gram positive and gram
negative selected bacteria.
The antimicrobial assay showed that the zones of inhibition produced using disc
diffusion method ranged 3mm to 22mm for the four extraction methods with the highest
value of 22mm obtained with ethanol extraction. The zones of inhibition for fresh
ethanol extract was 4 to 22 mm, methanol extract from fresh leaf was 8 to 20 mm, and
hot and cold water extract of fresh and dried leaves was 8 to 9,8 to 10,3 to 4 and 3 to 5
mm respectively. The MIC values were conducted for the different form of extracts
against the tested bacterial pathogens and the highest concentration was recorded
25mg/ml for S.auerus while it was 30 mg/ml for S.fecalis. The bacterial cultivated value
of M.oleifera powder extract of fresh leaf juice was determined by cultivating the four
tested pathogens on the culture medium which was prepared from the plant extract with
the proper concentration of agar powder in sterile distilled water and it was revealed
that all the pathogens were successfully grown on it. The consequences of this
investigation suggest that the extracts and juice of M.oleifera Lam. can be used to
discover antibacterial agent for developing new pharmaceuticals to control animal
pathogenic bacteria responsible for severe illness.
Description
Keywords
Antimicrobial, leaf, Moringa oleifera, Pathogenic bacteria