Bacteriological quality and antimicrobial resistance patterns of isolates from raw milk at selected dairy farms in Ethiopia.

Simple item page
dc.contributor.advisorDesta,Kassu(Ass.Prof.)
dc.contributor.advisorAbera,Dessie (PhD candidate)
dc.contributor.authorSebsibe, Kaleab
dc.date.accessioned2024-11-28T10:10:17Z
dc.date.available2024-11-28T10:10:17Z
dc.date.issued2023-07
dc.description.abstractBackground: Food-borne illness affects up to 30% of the population in developing countries each year, with great impact on public health, and the economy. Milk-borne infections cause acute, and severe disease. However, in developing countries there is limited information regarding bacterio- logical status of raw milk. This study aimed to assess the bacteriological quality and antimicrobial resistance patterns of isolates from raw milk. Objective: To evaluate the bacteriological quality and antimicrobial resistance patterns of isolates from raw milk at different dairy farms found in Ethiopia. Methods: A cross-sectional study design was conducted from August 2022–April 2023.A total of 176 raw milks samples were selected using multi-stage sampling technique. Bacteriological tests such as aerobic colony count, total coliform, thermotolerant coliform, Salmonella and Shigella detection, and Staphylococcus enumerations were evaluated. VITEK-2 compact was used for fur- ther bacterial identification, while antimicrobial susceptibility test was determined by disc-diffu- sion techniques. SPSS V.27 was use for data analysis; descriptive statistics were used. Result: The mean aerobic colony count, total coliform, thermo-tolerant coliform, E. coli, and Staphylococcus count was,3.2, 2.70, 2.69, 2.9, and 3.1 log10 cfu/ml respectively. E. coli (77%), Klebsiella spp. (5%), Salmonella spp. (1.4%), S. aureus (13.6%), S. saprophyticus (9.8%) were also isolated. Gram-negative isolates showed sensitivity for Meropenem (96.8%) and Ciprofloxa- cin (89.9%) but resistant against Tetracycline (62.2%) and Streptomycin (62.2%). Staphylococcus isolates showed 95.1 % Sensitivity for Levofloxacin and Gentamycin but 92.6 % of isolates were resistant against Oxacillin. Extended-spectrum beta-lactamase production was detected in 16.6% of gram-negative isolates, and Methicillin resistance were observed in 30% of S. aureus isolates. Conclusion: The result demonstrated, the overall bacteriological quality of raw milk is poor which also contains pathogenic and antibiotic-drug resistant organisms; thus, the need for effective con- trol measures to improve production, storage, and handling practices is implied.
dc.identifier.urihttps://etd.aau.edu.et/handle/123456789/3781
dc.language.isoen_US
dc.publisherAddis Ababa University
dc.subjectRaw milk
dc.subjectDairy farms
dc.subjectBacteriological quality
dc.subjectAMR.
dc.titleBacteriological quality and antimicrobial resistance patterns of isolates from raw milk at selected dairy farms in Ethiopia.
dc.typeThesis

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Kaleab Sebsib.pdf
Size:
647.57 KB
Format:
Adobe Portable Document Format
Download

Collections

Medical Laboratory Sciences