Production and Optimization of Bio-Ethanol from Avocado Seed
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Date
2016-06-06
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Publisher
AAU
Abstract
This study employs powdered avocado seed wastes as a raw material for the production of
Bioethanol. It was used dilute acid pretreatment, dilute acid hydrolysis, fermentation and
distillation processes. The experiment was designed by Central Composite Design (CCD) with
three factors (acid concentration, temperature and time) and triplicate run, the acid
concentration varied from 1.5 to 3.5%, hydrolysis temperature varied from 125 to 145℃ and
hydrolysis time was varied from 30 to 90 minutes. The different parameters of hydrolysis
conditions were optimized. The effect of hydrolysis temperature, hydrolysis time and acid
concentration on glucose yield was studied. The maximum yield of glucose was achieved at 60
minutes hydrolysis time, acid concentration of 1.5% and hydrolysis temperature of 135℃ with
maximum yield of 49.60% under optimized conditions. Acid concentration and hydrolysis time
have a statistically significant effect on the yield with p-value of 0.0016 and 0.0320 respectively.
Higher acid concentration as well as increased hydrolysis temperature causes a decline in the
glucose yield. Chemical characterization of the bio-ethanol produced was performed by FTIR.
The result shows that, the ethanol produced contains OH, CO, CH
viii
2
, and CH
3
functional groups
which indicate the presence of ethanol in the product.
Description
Keywords
Bio-Ethanol, Avocado Seed, Optimization