Production and Optimization of Bio-Ethanol from Avocado Seed

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Date

2016-06-06

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Publisher

AAU

Abstract

This study employs powdered avocado seed wastes as a raw material for the production of Bioethanol. It was used dilute acid pretreatment, dilute acid hydrolysis, fermentation and distillation processes. The experiment was designed by Central Composite Design (CCD) with three factors (acid concentration, temperature and time) and triplicate run, the acid concentration varied from 1.5 to 3.5%, hydrolysis temperature varied from 125 to 145℃ and hydrolysis time was varied from 30 to 90 minutes. The different parameters of hydrolysis conditions were optimized. The effect of hydrolysis temperature, hydrolysis time and acid concentration on glucose yield was studied. The maximum yield of glucose was achieved at 60 minutes hydrolysis time, acid concentration of 1.5% and hydrolysis temperature of 135℃ with maximum yield of 49.60% under optimized conditions. Acid concentration and hydrolysis time have a statistically significant effect on the yield with p-value of 0.0016 and 0.0320 respectively. Higher acid concentration as well as increased hydrolysis temperature causes a decline in the glucose yield. Chemical characterization of the bio-ethanol produced was performed by FTIR. The result shows that, the ethanol produced contains OH, CO, CH viii 2 , and CH 3 functional groups which indicate the presence of ethanol in the product.

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Keywords

Bio-Ethanol, Avocado Seed, Optimization

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