Production of Poly-hydroxybutyrate (PHB) from Glucose Derived from Sugar Cane Bagasse using Bacillus Subtilius
| dc.contributor.advisor | Zebene, Kiflie (PhD) | |
| dc.contributor.author | Tinsae, Messay | |
| dc.date.accessioned | 2021-04-29T06:45:06Z | |
| dc.date.accessioned | 2023-11-28T13:30:27Z | |
| dc.date.available | 2021-04-29T06:45:06Z | |
| dc.date.available | 2023-11-28T13:30:27Z | |
| dc.date.issued | 2021-03 | |
| dc.description.abstract | Most plastic materials are produced from petrochemicals. However, due to their non-degradable nature, they are causing serious environmental problems. The objective of this study was to obtain a bio-plastic material known as poly-hydroxybutyrate (PHB) based on glucose extracted from sugarcane bagasse with the help of bacillus subtilius. under stressing media condition. The extraction process stages were pretreatment, hydrolysis, fermentation, extraction, and recovery. The first stage was pretreating sugar cane bagasse and hydrolysis using dilute sulfuric acid in 1:8 solid/liquid ratios. The three factors analyzed were temperature, pH, and fermentation time for extraction of PHB. Synthesized PHB was characterized using Sudan staining, UV spectrophotometer and FTIR. The Response Surface Methodology with Box-Behnken experimental design was used to find the optimum extraction conditions. The effects of extraction parameters (temperature, pH, and fermentation time) on the response (poly-hydroxybutyrate yield) were analyzed. The optimum PHB yield of 6.82 g/l (0.17 g/g of bagasse) of was obtained at 37.3 0c, 7.2 pH and 48.6 hours. From the statistical analysis, all the factors had significant effect on the yield. Moreover, the interaction effects of temperature and pH, temperature and fermentation time, and pH and fermentation time had also significant effects on the yield. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/26250 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | sugarcane bagasse | en_US |
| dc.subject | bacillus subtilius | en_US |
| dc.subject | poly-hydroxybutyrate (PHB) | en_US |
| dc.subject | bio-plastic | en_US |
| dc.title | Production of Poly-hydroxybutyrate (PHB) from Glucose Derived from Sugar Cane Bagasse using Bacillus Subtilius | en_US |
| dc.type | Thesis | en_US |