Establishment of Serum Lipid Profile and Electrolytes Reference Intervals for Apparently Healthy Children and Adolescents in Addis Ababa, Ethiopia
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Date
2020-07
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Addis Abeba University
Abstract
Background: Reliable and accurate reference intervals for laboratory analyses are an integral partof correct interpretation of clinical laboratory test results. In Addis Ababa, Ethiopia, there were noreference intervals established on lipid profile and electrolytes which are essential in the assessment of early dyslipidemia and electrolyte abnormalities.
Objective: To establish reference intervals on serum lipid profile and electrolytes for apparentlyhealthy children and adolescents from April to October 2019 in Addis Ababa, Ethiopia.
Methods: Community based cross sectional study was conducted from April to October 2019. Atotal of 522 participants aged 5-17 years were recruited from communities. After physicalexamination, socio-demographic, anthropometric data and blood samples were collected. Serumlevels of lipid profile and electrolytes were determined using Cobas c501. In accordance withClinical and Laboratory Standards Institute guidelines reference intervals for four lipid profile andsix electrolytes were established by calculating 2.5th and 97.5th limits with 90% confidence intervalusing SPSS version 23.
Results: In children, the reference intervals for serum potassium, sodium, chloride, calcium,magnesium and phosphate in mmol/L were 4.37-5.2, 137-145.5, 101.9-107.9, 2.34-2.7, 0.74-0.97,and 1.42-1.85 and for total cholesterol, triglycerides, low density lipoprotein and high densitylipoprotein the respective values were 100.76-171.7, 44.16-126.36, 60.6-105.6 and 31.6-53.7 inmg/dl, for both sexes. For adolescents 4.03-5.58, 137-146, 98.9-120.9, 2.39-2.7, 0.73-0.96, 0.961.8forserum potassium,sodium,chloride,calcium,magnesium,phosphateinmmol/Land97.2189.1,40.5143.6,420.9and21.357inmg/dlfortotalcholesterol,triglycerides,lowdensitylipoproteinand high density lipoprotein,respectively,forboth sexes.
Conclusion: The obtained reference intervals by the current study revealed that both lower andupper limits were in disagreement with the manufacture as well as published literatures. The studyalso observed significant differences in the reference values between genders for all analytesexcept sodium, calcium and magnesium at the adolescents. Therefore, it is important to use thecurrent reference intervals.
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Reference intervals, lipid profile, electrolyte, children and adolescent