Extended Spectrum Beta-Lactamase Production and Multi-Drug Resistant Enterobacteriaceae Among Urinary Tract Infection Suspected Patients Referred to Wudassie Advanced Medical Laboratory, Addis Ababa, Ethiopia.
| dc.contributor.advisor | Desta,Kassu(MSc, PhD) | |
| dc.contributor.advisor | Diriba,Regasa(MSc) | |
| dc.contributor.author | Tamiru,Tamirat | |
| dc.date.accessioned | 2026-06-22T16:00:11Z | |
| dc.date.available | 2026-06-22T16:00:11Z | |
| dc.date.issued | 2025-06 | |
| dc.description.abstract | Background: Urinary tract infections (UTIs) are one of the commonly encountered infectious diseases globally, affecting approximately 150 million individuals each year. The rise of multidrug resistant (MDR) and extended spectrum beta lactamase (ESBL) producing Enterobacteriaceae has made the management of urinary tract infections more difficult which is becoming common public health concern worldwide Objective: To assess the prevalence, antimicrobial susceptibility, and extended-spectrum beta-lactamase producing Enterobacteriaceae among UTI suspected patients referred to Wudassie advanced medical laboratory, Addis Ababa, Ethiopia. Methods: A laboratory based cross-sectional study was conducted between December 2024 to March 2025. A total of 502 UTI suspected patients were included using convenient sampling technique. The identification of Enterobacteriaceae was done using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) (EXS3000, Zybio Inc., China). Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion technique. Combination disk test (CDT) was used for confirmation of ESBL producing Enterobacteriaceae. Data ware entered in Epidata version 4.6 and exported to Statistical Package for the Social Sciences (SPSS) version 27 for analysis. Result: Among 502 patients presenting with clinical symptoms of urinary tract infection (UTI), 29.4% (148/502) has positive culture for Enterobacteriaceae. The predominant species isolated were Escherichia coli 103(69.6%) and Klebsiella pneumoniae 25 (16.8%). The prevalence of extended spectrum β-lactamases producing Enterobacteriaceae (ESBLs-E) was 65.5% (97/148). The highest proportion of ESBL-producing isolate was Klebsiella pneumoniae (92.0%)..Among 148 Enterobacteriaceae isolated, 82.2% of them were multi-drug resistant (MDR). Conclusion: The findings highlight a high prevalence of MDR and ESBL-producing Enterobacteriaceae. This finding emphasizes a critical need to implement comprehensive surveillance systems in hospital settings to enable the detection and tracking of ESBL-producing Enterobacteriaceae. Continuous monitoring of their dissemination is highly recommended for implementation of antibiotic stewardship programs. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/8505 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Urinary tract infection | |
| dc.subject | Multi drug resistance | |
| dc.subject | Extended spectrum beta lactamase | |
| dc.title | Extended Spectrum Beta-Lactamase Production and Multi-Drug Resistant Enterobacteriaceae Among Urinary Tract Infection Suspected Patients Referred to Wudassie Advanced Medical Laboratory, Addis Ababa, Ethiopia. | |
| dc.type | Thesis |