Assessment of anti-tissue transglutaminase-IgG with selected lipid panel tests among Helminths and H.pylori positive and non infected school aged children in Ziway Oromia region, South East Ethiopia
| dc.contributor.advisor | Dr.Tsegaye, Aster(MSc, PhD, Associate Professor) | |
| dc.contributor.advisor | Dr.Wolde, Mistre(MSc, PhD, Associate professor) | |
| dc.contributor.advisor | Dr.Taye, Binyam(MSc, PhD, Assistant professor) | |
| dc.contributor.author | Alem, Mekdes | |
| dc.date.accessioned | 2020-12-16T11:32:17Z | |
| dc.date.accessioned | 2023-11-06T08:56:43Z | |
| dc.date.available | 2020-12-16T11:32:17Z | |
| dc.date.available | 2023-11-06T08:56:43Z | |
| dc.date.issued | 2020-07 | |
| dc.description.abstract | Background: Celiac disease (CD) is an autoimmune disorder triggered by consuming a protein called gluten. It is determined by both environmental and genetic factors. Infection by Helminths and H.pylori are among the environmental factors involved that enhance anti-inflammatory response. Different auto antibodies and antibodies are used in the diagnosis of celiac disease, but Anti-tTG antibodies are highly sensitive and specific for the diagnosis of CD. IgG-based tests are useful for detecting celiac disease. Since Celiac disease is one of the areas that did not get much attention in its diagnosis; this study could give some insight on the necessity of doing more research in the area and gives a better understanding on the association between celiac diseases with that of lipid profiles, H.pylori and Helminths infection Objective: The objective of this study was to assess anti-tissue transglutaminase IgG antibody with selected lipid panels among H.pylori and Helminths positive and non infected Children. Methods: A cross sectional retrospective and prospective study was conducted in Ziway, South east of Addis Ababa, Ethiopia by using random sampling method. The retrospective part included taking serum samples that were collected and stored during another study and careful review of study groups sociodemographic and medical data. The samples that were kept refrigerated at -80ºC were used for analysis of anti tissue transglutaminase IgG and lipid profiles as for the prospective part of the study starting from May 2020 to June 2020. The transglutaminase IgG measurement used principle of Enzyme immunoassay for optical density measurement based on commercially available kit. While the lipid panels used kinetic assay that were measured on Cobas 6000 fully automated chemistry machine. The obtained data was entered and analyzed by using SPSS version 23 and descriptive analysis, Chisquare and logistic regression were used to see any associations and P-value <0.05 was considered as statistical significant. Result: A total of 97 samples of children with age group between 4-14 years old were involved in this crossectional study. 19% of study participants tested positive for anti-tissue transglutaminase IgG and Females had a higher magnituse of anti-tTG IgG than males (24.5 % vs 11.4%). A significant association was found between Helminths and anti-tTGIgG [OR 3.45 (95% CI: 1.057, 11.265), P=0.033] However this study failed to show association between anti-tTGIgG and H.pylori as well as with lipid profiles. Conclusion: Although the relation between H. pylori and Celiac diseases is controversial, our result doesn’t proof H.pylori can shield against celiac disease so measures to protect oneself against H.pylori should be continued. On the other hand, even though more trials are required, there is a strong association between Helminthes infection and anti-tTGIgG where children not infected with Helminths had a 3.45 times greater risk of developing a positive antitTGIgG. Although a significant difference was not found in this study between lipid profiles and anti tissue transglutaminase, it is advisable to assess lipid profiles of celiac patients as shown in other studies so as to minimize the long term effect associated with lipid profile alteration, Coronary artery disease for instance. We strongly recommend large scale case control study to understand the exact relationship between celiac diseases and other comorbidities. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/24134 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | Celiac Disease, anti-tissue transglutaminase IgG, lipid panels. | en_US |
| dc.title | Assessment of anti-tissue transglutaminase-IgG with selected lipid panel tests among Helminths and H.pylori positive and non infected school aged children in Ziway Oromia region, South East Ethiopia | en_US |
| dc.type | Thesis | en_US |