Assessment of anti-tissue transglutaminase-IgG with selected lipid panel tests among Helminths and H.pylori positive and non infected school aged children in Ziway Oromia region, South East Ethiopia
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Date
2020-07
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Addis Abeba University
Abstract
Background: Celiac disease (CD) is an autoimmune disorder triggered by consuming a protein called gluten. It is determined
by both environmental and genetic factors. Infection by Helminths and H.pylori are among the environmental factors involved
that enhance anti-inflammatory response. Different auto antibodies and antibodies are used in the diagnosis of celiac disease, but
Anti-tTG antibodies are highly sensitive and specific for the diagnosis of CD. IgG-based tests are useful for detecting celiac
disease. Since Celiac disease is one of the areas that did not get much attention in its diagnosis; this study could give some insight
on the necessity of doing more research in the area and gives a better understanding on the association between celiac diseases
with that of lipid profiles, H.pylori and Helminths infection
Objective: The objective of this study was to assess anti-tissue transglutaminase IgG antibody with selected lipid
panels among H.pylori and Helminths positive and non infected Children.
Methods: A cross sectional retrospective and prospective study was conducted in Ziway, South east of Addis
Ababa, Ethiopia by using random sampling method. The retrospective part included taking serum samples that were
collected and stored during another study and careful review of study groups sociodemographic and medical data.
The samples that were kept refrigerated at -80ºC were used for analysis of anti tissue transglutaminase IgG and lipid
profiles as for the prospective part of the study starting from May 2020 to June 2020. The transglutaminase IgG
measurement used principle of Enzyme immunoassay for optical density measurement based on commercially
available kit. While the lipid panels used kinetic assay that were measured on Cobas 6000 fully automated chemistry
machine. The obtained data was entered and analyzed by using SPSS version 23 and descriptive analysis, Chisquare
and logistic regression were used to see any associations and P-value <0.05 was considered as statistical
significant.
Result: A total of 97 samples of children with age group between 4-14 years old were involved in this crossectional
study. 19% of study participants tested positive for anti-tissue transglutaminase IgG and Females had a higher
magnituse of anti-tTG IgG than males (24.5 % vs 11.4%). A significant association was found between Helminths
and anti-tTGIgG [OR 3.45 (95% CI: 1.057, 11.265), P=0.033] However this study failed to show association
between anti-tTGIgG and H.pylori as well as with lipid profiles.
Conclusion: Although the relation between H. pylori and Celiac diseases is controversial, our result doesn’t proof
H.pylori can shield against celiac disease so measures to protect oneself against H.pylori should be continued. On
the other hand, even though more trials are required, there is a strong association between Helminthes infection and
anti-tTGIgG where children not infected with Helminths had a 3.45 times greater risk of developing a positive antitTGIgG.
Although a significant difference was not found in this study between lipid profiles and anti tissue
transglutaminase, it is advisable to assess lipid profiles of celiac patients as shown in other studies so as to minimize
the long term effect associated with lipid profile alteration, Coronary artery disease for instance. We strongly
recommend large scale case control study to understand the exact relationship between celiac diseases and other comorbidities.
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Keywords
Celiac Disease, anti-tissue transglutaminase IgG, lipid panels.