Bioaccumulation of Cr(VI) from Tannery Wastewater Using Saccharomyces Cerevisiae
| dc.contributor.advisor | Shegaw, Ahmed (Assoc.Prof.) | |
| dc.contributor.author | Tessafa, Abrham | |
| dc.date.accessioned | 2020-11-18T07:29:20Z | |
| dc.date.accessioned | 2023-11-28T13:30:26Z | |
| dc.date.available | 2020-11-18T07:29:20Z | |
| dc.date.available | 2023-11-28T13:30:26Z | |
| dc.date.issued | 2018-06 | |
| dc.description.abstract | Chrome tanning is the most and widely used types of tanning process and the wastewater generated by these industries is a major source of chromium pollution in the form of Cr(III)&Cr(VI) which are released into the environment either after treated inefficiently or with no treatment and become a cause for many human health problem. Several researchers have published papers to find the solution for removal of Cr(VI) but at its low concentration and this research work investigates at high concentration of Cr(VI). Cell suspensions of S.cerevisiae at its exponential growth phase were added to 250ml plastic flasks of containing 50ml of YPD media at pH value of (3,5&8), initial Cr(VI) concentration of (5, 50&100mg/ml) and incubation time of(3,5&7 days) and shaken at 200rpm an from tannery wastewater using S.cerevisiae shows good result after Cr(VI)-contaminated wastewater has been treated at different treatment variables of pH, initial Cr(VI) concentration. Cr(VI) uptake efficiency of S.cerevisiae increases at low values of pH, Cr(VI) and incubation time and decreases at high values of these variables. And it can be conclude that Cr(VI)uptake efficiency is better at low values of the variables and the optimum conditions obtained by using the full factorial experimental design are pH value of 4.5, Cr(VI)concentration of 5mg/ml and incubation time of 5 days resulting 84.25 % theoretical Cr(VI) uptake efficiency . Cell viability test assay of this work shows the possibility of Cr (VI) removal at its high concentration using living cells that are tolerant to high concentration though the efficiency decreases as concentration increases due to inhibition. Bioaccumulation of Cr(VI) by the cell S.cerevisiae is analyzed by FTIR by comparing their IR spectrum of functional groups of the Cr(VI) free and Cr(VI) loaded S.cerevisiae cell wall that showed change of wavenumbers of biocomponents of cell wall of the two cells which confirms the binding of chromium ion with the functional groups of cell wall of S.cerevisiae and produces Cr complex compounds /precipitates. Freundlic equilibrium and pseudo second order kinetic model are used for expressing | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/23363 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Tannery Wastewater | en_US |
| dc.subject | Hexavalent Chromium | en_US |
| dc.subject | Bioaccumulation | en_US |
| dc.subject | FTIR analysis | en_US |
| dc.subject | Scerevisiae yeast cell | en_US |
| dc.title | Bioaccumulation of Cr(VI) from Tannery Wastewater Using Saccharomyces Cerevisiae | en_US |
| dc.type | Thesis | en_US |