Prevalence and Antimicrobial Susceptibility of Entrococcus Species Isolated from Different Clinical Samples at Tikur Anbesa Hospital Addis Ababa, Ethiopia
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Date
2016-05
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Addis Ababa University
Abstract
Background: Enterococci which are part of the normal intestinal flora are opportunistic human
pathogens. The two most important species, E.faecalis and E.faecium are among the leading
causes of nosocomial infections that may cause severe infections including endocarditis, urinary
tract infection, septicemia and wound infections which are often difficult to treat. Their
increasing importance is largely due to their resistance to antimicrobials. So the aim this study is
to determine the prevalence and antimicrobial pattern of Entrococcus spp.
Objective: To determine the prevalence and antimicrobial susceptibility of Entrococcus species
isolated from different clinical samples at Tikur Anbesa Specialized Hospital Addis Ababa,
Ethiopia .
Method: A cross sectional study was conducted from April to May 2016 on patients visiting
Tikur Anbesa Specialized Hospital. A total of 422 patients were included in the study. Clinical
samples(blood, urine, pus, bodyfluid and csf) was inoculated onto blood agar and mackonky
culture media .The isolates were identified by cultural characteristics, Gram’s stain, catalase
test, growth in 6.5% NaCl broth, bilesculin test, PYR test. Drug susceptibility pattern of the
isolates were determined using the Kirby Bauer disc diffusion method. Confirmation of
vancomycin susceptibility was done by the Epsilometer test to determine the Minimum
Inhibitory Concentration. Results obtained from the study was entered to excel spreadsheet and
transport to and analyzed using SPSS version 20 to test for statistical associations. P values less
than 0.05 were considered as significant.
Result:From a total of 422 sample processed 15 Entrococcus spp were isolated .Among the
isolate 53.3% were female and 46.7 %were male patients . In this studyLizolide were the drug of
choice for Entrococcus spp 100% sensitive followed by Vancomycen 93.3% sensitive in
contrast Ampcillen was highly resistance by 80% followed by Doxicyclin 73.3% resistance.
Conclusion: This study showed that the rate of isolated Enterococci was 3.5% and had variable
degrees of resistance to the antibiotics, but all were sensitive to Linzolide by disc diffusion
methods so the presence of VREand multidrug resistant Enterococci in our study should be
considered as an alarm for serious Enterococcal infections .
Key words: Entrococcus, Nosocomial infection, clinical samples , Antibiotic susceptibility
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Keywords
Entrococcus, Nosocomial infection, Clinical samples