Parametric Optimization and Characterization of Ferulic Acid Produced from Brewery Spent Grains
| dc.contributor.advisor | Anuradha, Jabasingh (PhD) | |
| dc.contributor.author | Hagos, Kalu | |
| dc.date.accessioned | 2020-03-06T10:45:34Z | |
| dc.date.accessioned | 2023-11-10T15:01:24Z | |
| dc.date.available | 2020-03-06T10:45:34Z | |
| dc.date.available | 2023-11-10T15:01:24Z | |
| dc.date.issued | 2019-11 | |
| dc.description.abstract | The utilization of agro-industrial wastes for the extraction of natural antioxidants such as Ferulic acid is currently getting a growing interest by the researchers around the world. This research study, therefore, aims at optimizing and characterizing the Ferulic acid extracted from brewery spent grains (BSG). Brewery spent grain was first treated with sulphuric acid before it was subjected to alkaline hydrolysis. The X-ray diffraction analysis of the raw and hydrolyzed BSG showed the evidence for a possible release of phenolic extractives after the hydrolysis. The effect of extraction time, extraction temperature, and alkali concentration on the Ferulic acid yield was studied at (60, 90, and 120 °C), (100, 120 and 140 min), and (1, 2 and 3% (w/v)) respectively. Optimization of these extraction parameters was carried out by the Box Behnken Design, using the Response Surface Methodology (Design experiment software version 11). It was observed that the Ferulic acid yield at different runs ranged from 6.62 mg/100 g BSG to 45.245±0.585 mg/100 g BSG. An optimum Ferulic acid yield of 46.85 mg/100 g BSG was observed at 119.83 °C, 97.04 min, and 2.21% (w/v) NaOH concentration. Both the Fourier Transform Infrared Spectroscopy (FT-IR) and High-Performance Liquid Chromatography (HPLC) analysis showed that the BSG was a good candidate for the extraction of the Ferulic acid. All the three main factors considered for this research study, namely the extraction time, extraction temperature and sodium hydroxide concentration had a statistically significant effect on the yield of Ferulic acid extracted. Spectrophotometric analysis of the produced Ferulic acid using DPPH radical scavenging method showed a strong capacity of its antioxidant activity. The result of the present research provides an alternative insight into the waste management of brewery spent grains (BSG) through the production of Ferulic acid. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/20952 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Ferulic acid | en_US |
| dc.subject | Brewery spent grains | en_US |
| dc.subject | Antioxidant | en_US |
| dc.subject | Extraction | en_US |
| dc.subject | Response surface methodology | en_US |
| dc.subject | Box Behnken Design | en_US |
| dc.subject | Optimization | en_US |
| dc.title | Parametric Optimization and Characterization of Ferulic Acid Produced from Brewery Spent Grains | en_US |
| dc.type | Thesis | en_US |