Production of Ethanol from Crude Glycerol Obtained from Transesterification Process Using Eschericha Coli’’
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Date
2017-07
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Addis Ababa University
Abstract
As a by-product of biodiesel production, glycerol has now become an abundant and cheap source of carbon. Conversion of this glycerol to higher value products will increase the economic viability of the biodiesel production process. The aim of this project work was to produce Bio-ethanol using crud and purified glycerol obtained from by-product of biodiesel production. A full factorial experimental design was used to investigate the effects of glycerol concentration, inoculum concentration and agitation speed on the production of ethanol via pure and crude glycerol by means of fermentation using Escherichia coli 0157:H7. Escherichia coli 0157:H7 was obtained from Ethiopian Biodiversity institute, Addis Ababa, Ethiopia and the crude glycerol were obtained from the transesterification process of soybean oils. Chemicals and reagents used were bought from local market (Niway PLC, Addis Ababa). The production of ethanol from purified and crude glycerol was compared. Maximum ethanol yield was obtained with a crud glycerol concentration of 10 g/L was 0.38 g/g glycerol under 7.5 % (v/v) of inoculum and 75 rpm of agitation speed and 0.42 g/g of glycerol under 10 % (v/v) of inoculum and 150 rpm of agitation speed. The predicted optimum ethanol yield 0.361252 was observed at the process variables 10 g/L glycerol concentration, 85.57 rpm speed of agitation and 8.8 % (v/v) of inoculum concentration. Keywords: Bioethanol, Biofuel, Transesterification, Fermentation and Escherichia coli
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Bioethanol; Biofuel; Transesterification; Fermentation and Escherichia coli