Bacterial Etiology of Bloodstream Infections, Multidrug Resistance and Extended beta Lactamase Producing Isolates among Patients Referred to Arsho Advanced medical Laboratory.
| dc.contributor.advisor | Dr.Bitew, Adane(PhD, Associate professor) | |
| dc.contributor.author | Abera, Misgana | |
| dc.date.accessioned | 2021-11-17T08:55:20Z | |
| dc.date.accessioned | 2023-11-06T08:57:02Z | |
| dc.date.available | 2021-11-17T08:55:20Z | |
| dc.date.available | 2023-11-06T08:57:02Z | |
| dc.date.issued | 2021-09 | |
| dc.description.abstract | Background: Blood stream infection is one of the most important causes of morbidity and mortality globally. Staphylococcus aureus, coagulase negative staphylococus, Streptococcus pneumonia are major Gram- positive bacteria in causing blood stream infection. Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii are major Gram-negative bacteria in causing blood stream infections. Development of multidrug resistance in major bacteria has increased the morbidity and mortality rate of blood stream infection. Objective: To determine bacterial etiology of blood stream infection, the prevalence of multidrug resistance,XDR, Pan drug resistance and extended spectrum beta lactamase production of bacterial isolates.Methods:The present study was a laboratory based cross sectional study conducted at Arsho Advanced Medical Laboratory, Addis Ababa Ethiopia from Dec 2020 to June 2021.A total 0f 422 blood sample was collected and inoculated onto primary isolation following standards protocols. Species identification and antimicrobial susceptibility testing of bacteria were determined by automated Vitek 2 compact system (Bio Merieux,France) by using AST,GN72 card for gram negatives and AST,GP71 card for gram positive bacteria. Results: Out of a total 422 samples processed, bacterial pathogens were isolated from 67(16%) samples. Among the isolates,40(59.7) were gram-negative and 27(40.3) were gram-positive bacteria. Klebsiella spp and Coagulase negative staphylococci were the dominat isolates. Of these isolates, 6 (60%) were ESBL positive for K.pneumonae,1(50%) for K.oxytoca and 4(66.7%) for E.coli. Penicillin(90.2%) was the least effective antibiotic against Gram-positive bacteria while Ampicillin(87.5%) were the least effective antibiotic against Gram-negative bacteria.Piperacillin(92.5%) were the most effective antibiotic against Gram-negative and Tigecycline(91.7%) were the most effective antibiotic against Gram-positive bacteria. Out of 40 isolate of Gram-negative bacteria 21(52.5%) were MDR and from 27 isolates of Gram-positive bacteria16 (59.3%) were MDR.Conclusion: The magnitude of blood stream bacterial infection and the prevalence rate of multi-drug resistant bacterial strains causing blood stream infections were high. These findings were warranted the need for the continuous investigation of bacterial blood stream infection. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/28729 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | Antimicrobial resistance, Multidrug resistance and Antimicrobial susceptibility. | en_US |
| dc.title | Bacterial Etiology of Bloodstream Infections, Multidrug Resistance and Extended beta Lactamase Producing Isolates among Patients Referred to Arsho Advanced medical Laboratory. | en_US |
| dc.type | Thesis | en_US |