The Effects of Chronic Treatment with DODONAEA ANGUSTIFOLIA Seed Extracts on some Hematological and Biochemical Composition of Blood and Histopathology of Liver and Kidney in Mice.
| dc.contributor.advisor | Dr.Kinfu, Yamrot | |
| dc.contributor.advisor | Makonnen, Eyasu(Prof.) | |
| dc.contributor.advisor | Dr.Ergete, Wondwossen | |
| dc.contributor.advisor | Urga, Kelbessa | |
| dc.contributor.author | Geneti, Soressa Abebe | |
| dc.date.accessioned | 2021-06-17T11:48:06Z | |
| dc.date.accessioned | 2023-11-29T04:16:20Z | |
| dc.date.available | 2021-06-17T11:48:06Z | |
| dc.date.available | 2023-11-29T04:16:20Z | |
| dc.date.issued | 2010-06 | |
| dc.description.abstract | In Ethiopia many medicinal plants are used for the treatment of malaria including Dodonaea angustifolia seed with out considering their side effects. Therefore, the present study attempts to evaluate the chronic effect of crude extracts of D. angustifolia seeds on some hematological and biochemical parameters and histopathology of liver and kidney of mice. For the chronic treatment, animals were administered with the crude extracts of the plant through the oral route using oral gavage for six weeks at doses of 400 mg/kg/bw and 800 mg/kg/bw of the aqueous extract and 400 mg/kg/bw and 600 mg/kg of the methanolic extract. At the end of the experiment, all mice under treatment were sacrificed after blood collection for hematological and biochemical analysis and liver and kidney sections were randomly taken for tissue processing. The results of hematological and biochemical analysis as well as the microscopic examinations of the liver and kidney sections were compared with control groups. The hematological and biochemical results showed no statistically significant differences between the aqueous extract treated mice at both doses of 400 mg/kg/bw and 800 mg/kg/bw and the control group. In contrast, statistically significant elevation in ALT, AST, urea and creatinine were observed in methanolic extract treated mice at a dose of 600 mg/kg/bw as compared to the control group. Histopathological examinations of the liver and kidney sections of aqueous crude extract treated mice at both doses of 400 mg/kg/bw and 800 mg/kg/bw showed similar histological appearance as that of the control group except some focal inflammation were observed around central vein at dose of 800 mg/kg/bw of the aqueous extract of the plant. In contrast, significant histopathological changes were observed in liver and kidney sections of methanolic crude extract treated mice at dose of 600 mg/kg/bw with focal cellular infiltration around central vein of the liver sections at 400 mg/kg/bw showing the dose dependent effect of methanolic crude extract of the plant. In conclusion, the aqueous crude extract is safe to use at effective dose of 400 mg/kg/bw whereas the methanolic crude extract of D. angustifolia at the same effective dose (400 mg/kg/bw) as that of aqueous crude extract has slight toxic effect but adversely affects the biochemical parameter and histology of liver and kidney as the dose level increases to 600 mg/kg/bw. As the aqueous extract did not show adversity at the effective dose, it is recommended that medicinal plant users choose the aqueous extract of the plant. Further investigation is also recommended to isolate metabolites that may contribute the toxic effect of methanolic crude extract on liver and kidney. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/26896 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Abeba University | en_US |
| dc.subject | Dodonaea angustifolia, chronic toxicity, liver, kidney, mice | en_US |
| dc.title | The Effects of Chronic Treatment with DODONAEA ANGUSTIFOLIA Seed Extracts on some Hematological and Biochemical Composition of Blood and Histopathology of Liver and Kidney in Mice. | en_US |
| dc.type | Thesis | en_US |