Epidemiology of Bovine Tuberculosis in East Wollega Zone, Western Ethiopia
| dc.contributor.advisor | Yasmin Jibril | |
| dc.contributor.author | Mezene Woyessa | |
| dc.date.accessioned | 2026-08-12T09:23:54Z | |
| dc.date.available | 2026-08-12T09:23:54Z | |
| dc.date.issued | 2010-06-01 | |
| dc.description.abstract | A cross-sectional study was conducted at Nekemte Abattoir and Horro-Guduru Cattle Breeding and Improvement Centre (HGCBIC) from September 2009 to May 2010 to estimate the prevalence of bovine tuberculosis (BTB), and characterize its causative agents using post mortem examination, comparative interadermal tuberculin (CIDT) test, bacteriological culturing, Zeihl Neelsen staining, multiplex polymerase chain reaction (PCR), and region of difference-4 (RD4) deletion typing. To accomplish these objectives, 1,186 cattle were recruited into the study from Nekemete Abattoir and 500 cattle from HGCBIC. The prevalence of BTB was 5.9% (701186) at Nekemete Abattoir on the basis of gross lesion while it was 1.8% (9/500) at HGCBIC using ClDT test. There was no significant difference (X2=4. 75; P=0.079) in prevalence of BTB between Horro-Jersy cross breed and zebu (Horro) breed at HGCBIC. At Nekemete Abattoir, 70% of the gross lesion was detected in the thoracic cavity while 25% of the lesion was found in the abdominal cavity. Only 31.4% (22/70) of the suspicious lesions yielded colonies of which 19 were acid-fast positive. Further identification of these 19 isolates using multiplex PCR revealed that 17 isolates belong to the Genus Mycobacterium while the remaining two isolates did not show signal to the Genus. Of the 17 isolates that showed signal to the Genus Mycobacterium, 7 were members of Mycobacterium tuberclosis (M. tuberclosis) complex while the remaining 10 isolates were members of the non-M. tuberculosis complex. Further identification and characterization of the M.tuberclosis complex members using RD4 deletion typing identified four isolates with intact RD4 which could be either M. tuberculosis or M. africanum and three isolates with deleted RD4 thus confirmed to be M. bovis. In conclusion, TB lesions were caused by both the members of M. tuberculosis complex and the non-M. tuberclosis complex Mycohacteria. Hence, as the majority of the isolates were the non-M. tuberculosis complex members, the pathogenecity of these members in cattle needs further study | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/8844 | |
| dc.language.iso | en | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Mycobacterium tuberculosis complex | |
| dc.subject | pathology | |
| dc.subject | tuberculin test | |
| dc.subject | prevalence | |
| dc.subject | risk factors | |
| dc.subject | Cattle | |
| dc.subject | East Wollega | |
| dc.subject | Ethiopia | |
| dc.title | Epidemiology of Bovine Tuberculosis in East Wollega Zone, Western Ethiopia | |
| dc.type | Thesis |