Development and Evaluation of Antimicrobial Aloe Based Packaging Films

No Thumbnail Available



Journal Title

Journal ISSN

Volume Title


Addis Ababa University


This study was aimed at the development of antimicrobial packaging films, which is one of the most promising active packaging systems, from aloe and papaya leaves extracts with gelatine and glycerol. Three different extracts of papaya leaves were analyzed for their antimicrobial activities. Mechanically obtained papaya leaf extract showed the best result (12.10mm inhibition zone on S.typhi). Based on antimicrobial activity, film forming ability, transparency, and colour; Aloe debrana extract was found to be more appropriate for the development of antimicrobial packaging films than Aloe trichosantha extract. The antimicrobial activity of Aloe debrana extract was increased by the incorporation of papaya leaf extract up to 30%, above which it did not bring significant influence on most test organisms. As a result 70% Aloe debrana extract and 30% papaya leaf extract standard solution was prepared. Various concentrations of gelatine and glycerol were added to the standard solution for packaging films development. Films were evaluated for their antimicrobial activities, physicochemical, and mechanical properties. The antimicrobial activities of aloe based films were tested on E. coli, S. typhi, S.aureus, C. albicans, and F. xylarioides. All films exhibited inhibitory zones on the test microorganisms used in this study. A wide inhibition zone (6.52cm2) was observed on S. typhi growth whereas the least (4.20 cm2) was on C.albicans. Films were soluble in water with highest solubility (90.49%) for P1,1 (film with 1g glycerol and 1g gelatine) and lowest (44.57) for P0,2(film developed by adding only gelatine). Film solubility significantly increased as the concentration of glycerol increased and decreased as the concentration of gelatine increased. Film P0.5,2(with 0.5g glycerol and 2g gelatine) has showed maximum tensile strength (65MPa) where as the lowest (20MPa) was obtained by film P0.5,1(0.5g glycerol and 1g gelatine). Increasing gelatine concentrations significantly increases the tensile strength but glycerol has an opposite effect on the tensile strength. All the films were highly flexible and stretchable. Film P1,2(1g glycerol and 2g gelatine) showed the maximum elongation (180%) and the minimum elongation (89%) was obtained for P0.5,1. This study demonstrated the technological feasibility of developing antimicrobial packaging films from aloe and papaya leaves extracts. Keywords: Aloe extracts, Antimicrobial packaging film, Mechanical properties, Papaya extracts, Pathogenic microorganisms, Physicochemical properties



Aloe extracts; Antimicrobial packaging film; Mechanical properties; Papaya extracts; Pathogenic microorganisms; Physicochemical properties