Production of Lactic Acid from Sugarcane Bagasse
| dc.contributor.advisor | Zebene, Kifle (PhD) | |
| dc.contributor.author | Mahamedberhan, Edris | |
| dc.date.accessioned | 2020-03-06T10:48:05Z | |
| dc.date.accessioned | 2023-11-10T15:01:24Z | |
| dc.date.available | 2020-03-06T10:48:05Z | |
| dc.date.available | 2023-11-10T15:01:24Z | |
| dc.date.issued | 2019-12 | |
| dc.description.abstract | Fossil fuel based plastic raw materials are not renewable which in questioned their sustainability as well as the plastic products of these raw material is sources of an overwhelming environmental problem due recalcitrant to degradation. Hence, there is an urgent need toward bio-based plastics research area particularly poly lactic acid (PLA). This study aimed to produce Lactic acid which is used as a raw material for bio-based plastic (PLA) by a bacteria Lacto bacillus plantarum using sugarcane bagasse as a carbon source. The effects of glucose concentration, time and pH on the lactic acid yield were studied. sugarcane bagasse was pretreated using dilute sulfuric acid as catalyst and a maximum glucose concentration of 3.755g/L was found at a temperature of 105 C, acid concentration of 2.75% and time of 3hrs.The fermentation experiments were performed in 250 mL Erlenmeyer flasks as small scale laboratory fermenter containing 100 mL of hydrolysate supplemented with 5g/L of yeast extract and 10mL of lacto-bacillus plantarum inoculum. The yield of lactic acid from the fermented broth was determined using HPLC. Response surface methodology (RSM) approach of Design expert software of version 11 was used to analyze the effects of glucose concentration (3.755g/L, 3.58.g/L and 3.42g/L), pH (4, 6 and 8) and incubation time (10hrs, 20hrs and 30hrs) on lactic acid production with their respective ranges keeping the temperature and agitation speed constant. A three-variable, three-level Box-Behnken design was used to develop a statistical model to describe the relationship between lactic acid concentration and the chosen independent variables and to observe their effects on the yield using RSM. The model was statistically significant (p ˂0.0001), did not show lack of fit (p-value = 0.1141) and The Pred R-Squared of 0.9764 is in reasonable agreement with the Adj R-Squared of 0.9956.From the model regression equation developed, the linear terms of glucose concentration, pH and time and pure quadratic term of time as well as interaction of glucose concentration and pH, interaction of glucose concentration and time, and interaction of pH and time had positive effect on the response yield. On the other hand pure quadratic terms of glucose concentration and pH had negative effect on the response yield. The maximum yield of lactic acid of 15.79g/L was found at a higher glucose concentration of 3.755g/L, at a moderate pH of 6 and at a maximum time of 30hrs. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/20953 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | bagasse | en_US |
| dc.subject | lactic acid | en_US |
| dc.subject | hydrolaysate | en_US |
| dc.subject | bio-plastic | en_US |
| dc.subject | Poly lactic acid | en_US |
| dc.title | Production of Lactic Acid from Sugarcane Bagasse | en_US |
| dc.type | Thesis | en_US |