Isolation and Identification of Corynebacterium Pseudotuberculosis from Superficial Lymphlinodes of Sheep and Goat at Organic Export Abbatoir, Modjo Ethiopia
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Date
2012-06
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Addis Ababa University
Abstract
Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CL.J\), a
disease characterized by the formation of suppurative abscesses, particularly in superficial and
internal lymph nodes, and in internal organs in small ruminants. The present study was conducted
from November to April 201 1/ 2012 only on the superficial lymph nodes and carcass of small
ruminants. Out of 768 small ruminants slaughtered at Organic slaughterhouse during the study
period, 82 (68 in goats and 14 in sheep) were found to have gross evidence of abscess or caseous
lymphadenitis. The typical gross lesion is a discrete abscess distended by thick and often dry,
greenish yellow or white, purulent exudates. The most frequent sites of abscesses in goats
occurred in the periscapular, pretemporal and popliteal lymph node and carcass 34(5.5'10/0).
24(3.91 %),2(0.33%) and 8(1.30%) respectively. In sheep 7(4.54%) in the periscapular, 5(3.25%)
in pretemporal and 0% in popliteal lymph node and 2(1.23%) abscess on carcass were found. The
study indicated that in both species young animals are more in number than adult, however,
caseous lymphadenitis was found to be higher in ad ult than young animals in both species. There
was statistically significant difference in the occurrence of CLA between age groups (P<0.05 J.
Out of 68 bacterial cutes from goats 18 and 50, pure and mixed colonies, respectively. livre
obtained; while out of 14 bacterial cultures boom sheep 4 and 10, pure and mixed colonies,
respectively, were obtained. Corynebacterium pseudotuberculosis isolates were obtained from S9
out of 82 collected abscessed lymph nodes and carcass. Bacterial colonies were identified on the
basis of morphological characteristics, primary and secondary biochemical tests, i.e cablese,
urease, trehalas, xylose, maltose, and glucose fermentation tests and reverse CAMP test
(antagonistic hemolysis between C. pseudotuberculosis and Staphylococcus aureus). Isolates
positive for catalase, urease, maltose, and glucose, but with inhibiting hemolysin of Soured'
by phospholipase D of C. pseudotuberculosis and negative for trehalas, and xylose "ere
considered as C. pseudotuberculosis. The susceptibility pattern of C. pseudotuberculosis to
antimicrobial agents varied among isolates. Out of 59 isolates of C. pseudotuberculosis isolated
from lesions of caseous lymphadenitis in goats and sheep were susceptible to the antibi optics
norfloxacin, tetracycline, doxycycline HCl and kanamycin.
Keywords: Antimicrobial agents; Caseous lymphadenitis; Corynebacterium pseudotuberculosis;
Goat; Lymph nodes; Mixed colonies; Pure colonies; Sheep.
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Keywords
Antimicrobial agents, Caseous lymphadenitis, Corynebacterium pseudotuberculosis, Goat, Lymph nodes, Mixed colonies, Pure colonies, Sheep.