Veterinary Pathology

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Browsing Veterinary Pathology by Subject "Biochemical changes"

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    Comparative Study on Pathological Changes in Sheep and Goats Experimentally Infected with Haemonchus Contortus
    (2020-06) Bethlehem, Mesfin; Dr. Jirata Shiferaw, Dr. Getachew Terefe
    The experimental study was done between October, 2019 and June, 2020 in the fly-proof experimental animal facility located in the premise of the college of veterinary medicine and Agriculture at Bishoftu. A total of 14 male goat (G1 and G2) and 14 male sheep (G3 and G4) were allotted in to four equal groups. Single dose of 10,000 of infective larvae of Haemonchus contortus (L3) was orally administered to each animal in G1 and G3. Parameters such as body weight, PCV, haemoglobin, worm count, serum total protein, serum albumin, alkaline phosphatase and aspartate aminotransferase were measured. Moreover, histopathological sections were stained and examined for general changes as well as for changes in specific cells such as tissue eosinophilia and parietal cell population. The findings show that 1) All infected sheep and goats developed the infection with higher mean worm burden in goats (5590) than sheep (2887) and the difference was significant (P<0.05); 2) All infected sheep and goats exhibited a progressive anaemia; the level being more severe in goats than in sheep (P< 0.05) with mean PCVs of 13% and 18.6% respectively; 3) While body weight gain was minimal in sheep, goats have lost significant weight compared to pre-infection levels, to control animals or in relation to infected sheep (P< 0.05); 4) analysis of biochemical changes also revealed marked reduction in serum total protein and albumin but again it was much more significant in goats than in sheep (P< 0.05); 5) infected sheep and goats‟ abomasa have shown thickening, nodule development, eosinophilic infiltration and damage to parietal cells. Tissue eosinophilia was more prominent in sheep while parietal cell loss was severe in goats. In conclusion, goats under experimental infection and similar management condition with sheep develop much more severe infection and associated pathology compared to sheep and hence deserve special attention.
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    Serum Biochemical, Haematological and Pathological Changes in Calves Experimentally Infected With t. Vivax Isolated From Tsetse and Non Tsetse Infected Areas of North West Ethiopia AfterTtreatment With Diminazen Aceturate and Isometamidium Chloride
    (Addis Ababauniversity, 2014-06) Tesfu, Frehiwet; Dr. Hailu, Yakob
    Longitudinal research on biochemical, haematological and histopathological changes in calves experimentally infected with T.vivax isolate from tsetse and non tsetse infested areas of North West Ethiopia after treatment with diminazene aceturate and Isometamidium chloride were conducted. Calves were grouped in to7 groups of 6 animals per group and then challenged by T. vivax at a dose of 1x106parasites/ml intravenously (group 1&2 challenged with isolate from tsetse area, group 3,4,5,6 with isolate from non tsetse area) and group 7(control animals) were received saline water. 14 days post infection calves in group1, group3 and group5 were treated with diminazene aceturate at a dose rate of 7mg/kg body weight and group2, group4 and group6 were treated with Isometamidium chloride at a dose rate of 1mg/kg body weight IM. All trypanosome-inoculated cattle developed acute form of trypanosomosis following the detection of parasitemia in the blood of infected calves. There was variable degree of reduction in mean PCV, Hb concentration and RBC after infection. After treatment all infected groups has resulted in significant improvements in which PCV, Hb concentration and total RBC counts became similar to the pre-infection values for group1 and group2. There was significant reduction in serum glucose, total protein and cholesterol and significant increase in serum enzymes in all challenged groups. After treatment all groups has resulted in significant improvements in the values which were become equivalent to or better than the pre-infection measurements. Gross and histopathological changes were observed in different tissues of calves after treatment with diminazene aceturate and Isometamidium chloride in all groups of calves which were highly xiv significant in calves infected with isolate from non tsetse area. In conclussion the result of the current experiment revealed that there were improvement after treatment but differences in haematological, biochemical and pathological parameters remained significant in all groups of calves treated with both drugs in comparison to the non-infected groups. So trypanocidal drugs which can not only result in clinical cure but also cause a better improvement in pathophysiological parameters should be developed. Key words: Biochemical changes, haematological changes, Pathological changes, Trypanocidal drugs, TT, NT