Medical Laboratory Sciences

Permanent URI for this collection

http://etd.aau.edu.et/handle/123456789/88

Browse

Browsing Medical Laboratory Sciences by Subject "Adolescent, Hematological parameter, reference interval"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    Establishment of hematological parameters reference intervals for apparently healthy adolescents in Asella town, South East Ethiopia
    (Addis Abeba University, 2020-06) Dibaba, Berhanu; Tsegaye, Aster (MSc, PhD); Alemu, Jemal(MSc)
    Background: -Physicians' medical decisions are based on information provided by laboratory reports in the form of a reference interval (RI) or medical decision limit. The interval serves as a health-related standard with which to compare an individual test result. However, the lack of bringing up as its own standard, local reference values has been a problem facing hematological practice in our country. Objective: To establish hematological parameters reference intervals for apparently healthy adolescents (12-17) in Asella town, South East Ethiopia from January to March 2020 GC. Method: A total of 342 apparently healthy adolescents aged 12–17 years living in Asella town and fulfilling the eligibility criteria were recruited for this cross-sectional descriptive study using a systematic random sampling method. Data including socio-demographic characteristics were collected using a structured questionnaire. The hematological analysis was performed using Sysmex KX-21 3 Diff analyzer. Data was entered and analyzed using SPSS version 21. The nonparametric Mann-Whitney u test was used to compare the parameters between genders. The 97.5 percentile and 2.5 th percentile as the upper and Lower Reference limit in favor of the adolescent population were determined. Result: The study observed statistically significant mean differences between males and females in Hemoglobin (HGB), Hematocrit (HCT), Mean cell hemoglobin concentration (MCHC), Mean Cell volume (MCV), absolute lymphocyte number (LYM#), Red Blood Cell (RBC), and Red blood cell distribution width by the coefficient of variation (RDWCV). Whereas the mean values of other Hematological parameters like WBC PLT and RWDSD have no significant difference between both sex (p value>0.05). Hence the reference interval of White Blood Cells (WBC), Platelet (PLT) and Red blood cell distribution width by the standard deviation (RWDSD) in adolescents were (3.410. 9x103/ul), (153.9-390x103/ul) and (36.8-47.1fl) respectively. The current established reference intervals show higher proportions out of range values when compared to the existing reference intervals. Conclusion: Some hematological parameter showed a significant difference in the mean values among data sets for HGB, RBC, MCV and MCHC across gender (p value<0.05) in which females having lower levels than males. The newly established hematological parameter reference intervals by this study were different from the existing reference values. th