Establishment of hematological parameters reference intervals for apparently healthy adolescents in Asella town, South East Ethiopia
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Date
2020-06
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Addis Abeba University
Abstract
Background: -Physicians' medical decisions are based on information provided by laboratory
reports in the form of a reference interval (RI) or medical decision limit. The interval serves as a
health-related standard with which to compare an individual test result. However, the lack of
bringing up as its own standard, local reference values has been a problem facing hematological
practice in our country.
Objective: To establish hematological parameters reference intervals for apparently healthy
adolescents (12-17) in Asella town, South East Ethiopia from January to March 2020 GC.
Method: A total of 342 apparently healthy adolescents aged 12–17 years living in Asella town and
fulfilling the eligibility criteria were recruited for this cross-sectional descriptive study using a
systematic random sampling method. Data including socio-demographic characteristics were
collected using a structured questionnaire. The hematological analysis was performed using
Sysmex KX-21 3 Diff analyzer. Data was entered and analyzed using SPSS version 21. The nonparametric
Mann-Whitney u test was used to compare the parameters between genders. The 97.5
percentile and 2.5
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percentile as the upper and Lower Reference limit in favor of the adolescent
population were determined.
Result: The study observed statistically significant mean differences between males and females
in Hemoglobin (HGB), Hematocrit (HCT), Mean cell hemoglobin concentration (MCHC), Mean
Cell volume (MCV), absolute lymphocyte number (LYM#), Red Blood Cell (RBC), and Red
blood cell distribution width by the coefficient of variation (RDWCV). Whereas the mean values
of other Hematological parameters like WBC PLT and RWDSD have no significant difference
between both
sex (p value>0.05). Hence the reference interval of White Blood Cells (WBC), Platelet (PLT) and
Red blood cell distribution width by the standard deviation (RWDSD) in adolescents were (3.410.
9x103/ul), (153.9-390x103/ul) and (36.8-47.1fl) respectively. The current established reference
intervals show higher proportions out of range values when compared to the existing reference
intervals.
Conclusion: Some hematological parameter showed a significant difference in the mean values
among data sets for HGB, RBC, MCV and MCHC across gender (p value<0.05) in which females
having lower levels than males. The newly established hematological parameter reference intervals
by this study were different from the existing reference values.
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Keywords
Adolescent, Hematological parameter, reference interval