Browsing by Author "Tsegaye, Aster ( PhD)"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Comparison of Cerebrospinal and Pleural fluid analysis using manual and automated hematology analyzer at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia.
    (Addis Abeba University, 2021-08) Aschenik, Mulualem; Tsegaye, Aster ( PhD); Alemu, Rahel(BSc, MSc); Mamushet, Yared(MD); Kebede, Henok ( MSc ); Tamire, Tewodros( MSc)
    Background: Analysis of body fluids provides important information for assessing various medical conditions. Bothe manual and automated hematology analyzers are used for cell counting. However, no published study is available to validate the diagnostic performance of the Sysmex 4000-i (Sysmex, Japan) system for the analysis of CSF and Pleural fluids. Objective: To compare the manual counts of cerebrospinal fluids (CSF) and pleural fluids (PF) with the Sysmex XT-4000i automated hematology analyzers. Methods: In this cross-sectional study, 240 body fluid samples were analyzed using the Sysmex 4000-i system in the body fluid mode and light microscopy at Tikur Anbessa Specialized Hospital. Body fluids included were pleural and cerebrospinal fluid (CSF). The results of the manual and automated counts were compared using the Pearson correlation test. Results: A comparison between the Sysmex 4000-i system and manual counting demonstrated great correlations with regard to pleural fluid WBC, Polymorph nuclear cells and Mono nuclear cell counts where r =0.82, 0.96, 0.95, respectively in both methods of the counts. Cell count from CSF also showed good correlations for both manual and automated counts of WBC, PMN % and MN% where r=0.83, 0.94, and 0.92, respectively. The Sysmex 4000-i system also demonstrated good performance for differential cell counting (r =0.9028). CSF particularly showed a good correlation. Conclusions: The use of the Sysmex 4000-i system for cell counting and differential analysis of body fluid samples might be an effective and automated alternative to chamber counting in laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.
  • No Thumbnail Available
    Item
    Sero-prevalence of HBsAgamong Pregnant Women and Rate of Vertical Transmission in Selected Health Facilities of Addis Ababa, Ethiopia
    (Addis Abeba University, 2021-07) Teklu, Abaynesh; Desta, Kassu(MSc, PhD candidate); Tsegaye, Aster ( PhD)
    Background: Pregnant women who are carriers of the HBV pose a significant risk to their health andnewborn babies. Majority of countries in developing world have not yet adopted universal screening for pregnant women.There are limited studies on maternal and their newborns HBV status in Ethiopia. Objective: - To determine the sero-prevalence of HBsAg among pregnant woman and rate of vertical transmission in selected health facilities of Addis Ababa, Ethiopia Methods: A prospective cross sectional studywasconductedat two hospitals from January– April, 2018 G/C. Sociodemographic data were collected using pretested structured questionnaire. Serawere collected to determine the HBSAg usingImmunochromatographic test kit andconfirmation by ELISA. Data entry and analyseswere done using SPSS 20 statistical software. Descriptive statistics were used to summarize data. Proportions and the corresponding 95% CI were presented. Mean and standard deviation wereused for numerical variables. Frequencies wereused for categorical variables. Bivariate analysis was carried out to see association between dependent and independent variables. P value of < 0.05 was considered significant associations. Results:A total of 420 samples from mothers and cord blood from newborns of positive mothers were collected. Of these 404(96.2%) lived in rural part of the country, 87 % (357)and 58.6 % (246) were married and have primary level education, respectively.Government workers accounted 20.7 % (87)of the study participants and 74.8% did not havehistory of blood transfusion. In this study 3 % (13)of pregnant women were vaccinated and 14,5 % (61)were in their first time pregnancy. Among 420 participants,2.38 % (10/420)were positive for HBV and no mother to child transmissionwas observed. Conclusion:Although the low prevalence ratein this study concurs with what has been categorized as intermediate endemicity and no vertical transmission is noted,every pregnant woman should be testedfor HBV and check mother to child transmission.