Browsing by Author "Tsegaye, Aster (MSc, PhD, Associate Professor)"

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    Establishment of Reference Interval for Hemoglobin A1c and Fructosamine for Apparently Healthy Adults in Addis Ababa, Ethiopia.
    (Addis Abeba University, 2020-07) Getahun, Tigist; Tsegaye, Aster (MSc, PhD, Associate Professor); Kinde, Samuel (MSc, PhD Candidate); Challa, Feyissa(MSc, PhD Candidate)
    Background: HbA1c and fructosamine used for diabetes monitor and control as a glycemic control. The absence of locally derived referenc intervals for these parameters from apparenetly health subjects for the local will impact on the physician, laboratories, and researchers. Objective: to establish reference interval for hemoglobin A1c and fructosamine for apparently healthy adults in Addis Ababa, Ethiopia. Methodology: A cross sectional study was conducted in four selected sub-cities (Akaki, Kirkos, Arada, Yeka), Addis Ababa, Ethiopia from December 2019 to April 2020. The study was including study participants with 18 and above years of age. Blood sample was collected and analyzed by using Cobas 6000(c 501) instrument. Both Kolmogorov–Sminorv, and MannWhitney test were analyzed to check the data normality distribution and partitions, respectively. Reference intervals, lower 2.5 th percentile and 97.5 th percentile for the upper, (overall, and specific for sex and age) were determined using non-parametric methods. Result: - Out of the total 344 participants, 256 (121 males, 135 females,) study participants aged 18-60 years participated in this study. Reference interval was established from 4.70 – 6.27% and 203 - 321mol/L for HbA1c, and fructosamine, respectively. There was weak association between HbA1c and age (r=0.1803, p=0.0378). Conclusion: This study established reference intervals for both HbA1c and Fructosamine for adult population using the current available equipment platform. The established reference interval is applicable for the current practice in the country to diagnosis, treatment, and monitor of diabetics mellitus.
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    Establishment of Reference Intervals for Selected Clinical Chemistry Parameters among Apparently Healthy Adults in Addis Ababa, Ethiopia
    (Addis Abeba University, 2020-06) Kassaw, Melkitu; Tsegaye, Aster (MSc, PhD, Associate Professor); Kinde, Samuel(MSc, PhD Candidate)
    Background: Reference interval values are used as medical decision-making tools that are given by clinical laboratory to assist health professionals, medical researchers for clinical trials and for physician to diagnosis, treatment, and follow-up of patients.. Objective: This study aimed to establish reference interval for selected clinical chemistry parameters among apparently healthy adults living in Addis Ababa, Ethiopia. Method: community based cross sectional study was conducted from March to October 2019 recruiting a total of 448 participants aged from 18-60 years. Medical history, physical examination, anthropometric and socio-demographic data were collected using structured questionnaire. Serum samples were analyzed for selected clinical chemistry parameters using Cobas 6000. Reference intervals were established by calculating 2.5 th -97.5 percentile with 90%Cl after excluding outliers using tukey method, Kolmogorov-Sminorv test. The difference between groups was computed using Mann-Whitney test by using SPSS version 23. P-value of <0.05 was taken as statistically significant. Result: In adults the reference interval of males and females were ALT 1.60-16.89U/l against females 0.47-9.87U/l, AST 8.11-22.50 vs.6.95-18.05 U/l, ALP 44.0-181.65U/l; Albumin 4.405.88U/l vs.4.03-5.25U/l, T.protein 6.76-9.57g/l vs. 6.45-8.21g/l, T.bilirubin 0.26-1.15 vs.0.150.64mg/dl, D. bilirubin 0.08-0.46mg/dl vs. 0.061-0.25mg/dl, Glucose 62.93-99.39mg/dl vs.59.14-90.72mg/dl, Urea 13.21-33.51mg/dl vs.7.30-12.00mg/dl, Creatinine 0.56-1.02mg/dl vs.0.40-0.80mg/dl and Uric acid 3.41-7.40mg/dl, vs.1.88-5.60mg/dl, respectively. Statistically significant differences were observed between males and females except Albumin and Total proteins. Conclusion: the reference interval for selected clinical chemistry parameters of adults was lower than the values given by manufacturers and Africans studies. Hence, it is more useful using locally established reference interval than derived from other countries. th