Browsing by Author "Alemu, Rahel(BSc, MSc)"

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    Assessment of hematological and biochemical alteration in type II diabetes mellitus patients attending Aksum St. Marry Hospital, Aksum, Northern Ethiopia
    (Addis Abeba University, 2021-09) Yhdego, Kidane; Tamir, Zemenu (MSc, PHD candidate); Alemu, Rahel(BSc, MSc)
    Background: Diabetes mellitus is a non-communicable disease that has a high prevalence worldwide. Altered level of many hematological and biochemical parameters have been observed in patients with diabetes. Although type ӀӀ diabetes mellites alters hematological and biochemical parameters, so far, very little work has been done in Ethiopia linking hematological parameters and biochemical markers with type ӀӀ diabetes mellites patients. Objective: To assess alteration of hematological and biochemical parameters in type ӀӀ diabetes mellitus patients attending Aksum St. Marry Hospital, Aksum, Northern Ethiopia, from December 2019-April 2020 Method: comparative cross-sectional study was conducted from December 2019 to April 2020 at Aksum St. Marry Hospital, Aksum, Northern Ethiopia. A total of 168 participants were enrolled using convenient sampling method. Socio-demographic, behavioral and physical measurement data were collected. Complete blood count was performed by sysmex KX-21N hematology analyzer. The biochemical parameters were analyzed by using BE200. Data was analyzed using SPSS 23. Student independent t-test and person’s correlation statistical method employed to analyze data. P values < 0.05 was regarded as statistically significant at 95% confidence level. Result: Among the hematological parameters WBC count, neutrophil count, lymphocytes count, platelet count, MPV significantly increased. Similarly, RBC count, hemoglobin and PCV showed significant decrease in T2DM patients compared to controls. With regard to biochemical parameters ALT, AST, ALP, Creatinine and urea showed significantly increasing in T2DM patients when compared to controls. Regarding correlation, WBC with BMI and MCHC with FBS had positive correlations. Conclusion: There is alteration in total WBC, neutrophil, lymphocyte, eosinophil, basophil, RBC, hemoglobin and PCV. Also, from biochemical parameters ALT, AST, ALP, urea and creatinine altered in diabetes patients compared with control group.
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    Comparison of Cerebrospinal and Pleural fluid analysis using manual and automated hematology analyzer at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia.
    (Addis Abeba University, 2021-08) Aschenik, Mulualem; Tsegaye, Aster ( PhD); Alemu, Rahel(BSc, MSc); Mamushet, Yared(MD); Kebede, Henok ( MSc ); Tamire, Tewodros( MSc)
    Background: Analysis of body fluids provides important information for assessing various medical conditions. Bothe manual and automated hematology analyzers are used for cell counting. However, no published study is available to validate the diagnostic performance of the Sysmex 4000-i (Sysmex, Japan) system for the analysis of CSF and Pleural fluids. Objective: To compare the manual counts of cerebrospinal fluids (CSF) and pleural fluids (PF) with the Sysmex XT-4000i automated hematology analyzers. Methods: In this cross-sectional study, 240 body fluid samples were analyzed using the Sysmex 4000-i system in the body fluid mode and light microscopy at Tikur Anbessa Specialized Hospital. Body fluids included were pleural and cerebrospinal fluid (CSF). The results of the manual and automated counts were compared using the Pearson correlation test. Results: A comparison between the Sysmex 4000-i system and manual counting demonstrated great correlations with regard to pleural fluid WBC, Polymorph nuclear cells and Mono nuclear cell counts where r =0.82, 0.96, 0.95, respectively in both methods of the counts. Cell count from CSF also showed good correlations for both manual and automated counts of WBC, PMN % and MN% where r=0.83, 0.94, and 0.92, respectively. The Sysmex 4000-i system also demonstrated good performance for differential cell counting (r =0.9028). CSF particularly showed a good correlation. Conclusions: The use of the Sysmex 4000-i system for cell counting and differential analysis of body fluid samples might be an effective and automated alternative to chamber counting in laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.