Browsing by Author "Alemu, Jemal(MSc, PhD candidate)"

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    Establishing Reference Interval for Hematological Parameters for Apparently Healthy Adult Men and Pregnant Women in Arbaminch, South Ethiopia
    (Addis Abeba University, 2021-09) Nedu, Adisu; Tsegaye, Aster (MSc, PhD); Alemu, Jemal(MSc, PhD candidate)
    Abstract Background: Hematological reference intervals (RIs) are important in routine assessments for the diagnosis of blood disorders, infectious diseases, immune diseases, diseases progression and assessments of ant-retroviral treatments. However, most of Africans including Ethiopia use RIs which are adopted from textbook, manufacturer of machines or Western countries while several factors are affecting these parameters. Objective: To establish hematological reference intervals for apparently healthy adult men and pregnant women at Arbaminch, Southern, Ethiopia, 2020/21. Methods: A cross-sectional study design was applied from Nov 2020 to Sep 2021. A systematic sampling technique was used to recruit a total of 312 men and pregnant women aged 18 years and above. Individuals who are apparently healthy(fulfill the eligibility criteria) and are voluntary to participate in the study were included. A structured questionnaire was used to collect data about socio-demographic, health status, drug usage, and nutritional habit of the participants after getting the ethical clearance. The laboratory specimens (blood, urine, stool) were collected according to standard operating procedures (SOPs) and the laboratory investigation was performed. Quality control material for hematological tests was used according to the SOPs. Age and sex specific 2.5 th and 97.5 th reference interval was determined using the non-parametric method. Data were entered and analyzed using SPSS version 23.0 software. Results:- The median and 95% RI for RBC parameters were, 4.92(4.32-5.79)*10 /L, 14.8(13.217.08)g/dl and 45.2(41.3-54.18)% for RBC, Hgb and Hct respectively for adult men and 4.39(3.565.16)*10 12 /L, 13(9.3-15.9)g/dl and 41(32.6-46.2)% for RBC, Hgb and Hct, respectively for pregnant women. The median and 95% RI for WBC parameters and platelet count were 7.5(4.14-11.5)*10 2.2(1.2-5.21)*10 9 /L, 0.7(0.3-1.2)*10 9 /L, 4.8(2.06-7.88)*10 9 /L, and 227(152-353.3)*10 /L WBC count, absolute lymphocyte, absolute mixed cell and absolute neutrophil and platelet count for adult men and 7.5(4.55-12.4)*10 9 /L, 2.2(1.2-3.9)*10 9 /L, 0.7(0.3-1.2)*10 9 /L, 4.9 (1.9-10.1)*10 /L and 202(142362)*10 9 /L WBC, Lymphocyte, Mixed cell, Granulocyte and platelet count for male and pregnant women, respectively. Conclusion:- The result from the current study identified that there is variation in most of the reference interval of hematological parameters especially red cell parameters with reference intervals conducted in other parts of the country, Africans as well as western countries.
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    Establishment of hematological reference intervals for apparently healthy adult residents in Asella town, southeast Ethiopia
    (Addis Abeba University, 2019-06) Tadesse, Solomon; Tsegaye, Aster(MSc, PhD); Alemu, Jemal(MSc, PhD candidate)
    Background: About 70% of medical decisions made by physicians are based on the information presented by laboratory results. However, test results by themselves are valueless unless reported with the appropriate reference interval or medical decision limit. Currently, Ethiopia use reference intervals adopted from textbooks that refer mainly to Caucasian subjects. The country having heterogeneous population, there is a need to establish locally derived hematological reference interval that could be used in Asella town, Arsi zone, Ethiopia. Objective: To establish hematological reference intervals for apparently healthy adults in Asella town, southeast, Ethiopia. Methods: A cross sectional study was conducted from January to March 2019 on apparently healthy individuals in Asella town aged from 18-60 years. Predesigned and structured questionnaire was used for collection of data on socio-demographic characteristics and dietary pattern of the reference population. Systematic random sampling technique was used. About 3ml of EDTA whole blood was collected and analyzed using Sysmex KX 21N automated hematology analyzer which analyses 60 tests per hour. The data was entered and analyzed by appropriate statistical software (Epi Info and SPSS) and interpreted using non-parametric methods, by which central 95% of the measured values was included in the intervals. Result: a total of 494 participants were recruited and 424 participants were involved in this study with the median age of 28 years. Except WBC (3.4 to 10.1 x10 9 /L) which showed no significant difference, other men and non-pregnant women reference intervals are: RBC (4.77 to 6.07 x10 /L; 4.18 to 5.29 x10 12 /L), HGB (14.7 to 18.1 gm/dL; 12.7 to 15.7gm/dL), HCT (42.1 to 51.3%; 37.1 to 44.4%) and PLT (159 to 336 x10 9 /L; 177 to 376 x10 9 /L), respectively. Pregnant women’s WBC, RBC, HGB and PLT are (4.9 to 13.2 x10 9 /L, 3.58 to 4.9 x10 12 /L, 11.0 to 14.6gm/dL and (138 to 368 x10 9 /L), respectively. Conclusion: most of the hematological RI of this study was significantly different from currently in use in Asella referral and teaching hospital laboratory. The difference was also observed in studies of other African countries as well as studies from different parts of Ethiopia. 12