Establishing Reference Interval for Hematological Parameters for Apparently Healthy Adult Men and Pregnant Women in Arbaminch, South Ethiopia
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Date
2021-09
Authors
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Journal ISSN
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Publisher
Addis Abeba University
Abstract
Abstract
Background: Hematological reference intervals (RIs) are important in routine assessments for the
diagnosis of blood disorders, infectious diseases, immune diseases, diseases progression and assessments
of ant-retroviral treatments. However, most of Africans including Ethiopia use RIs which are adopted
from textbook, manufacturer of machines or Western countries while several factors are affecting these
parameters.
Objective: To establish hematological reference intervals for apparently healthy adult men and pregnant
women at Arbaminch, Southern, Ethiopia, 2020/21.
Methods: A cross-sectional study design was applied from Nov 2020 to Sep 2021. A systematic
sampling technique was used to recruit a total of 312 men and pregnant women aged 18 years and above.
Individuals who are apparently healthy(fulfill the eligibility criteria) and are voluntary to participate in the
study were included. A structured questionnaire was used to collect data about socio-demographic, health
status, drug usage, and nutritional habit of the participants after getting the ethical clearance. The
laboratory specimens (blood, urine, stool) were collected according to standard operating procedures
(SOPs) and the laboratory investigation was performed. Quality control material for hematological tests
was used according to the SOPs. Age and sex specific 2.5
th
and 97.5
th
reference interval was determined
using the non-parametric method. Data were entered and analyzed using SPSS version 23.0 software.
Results:- The median and 95% RI for RBC parameters were, 4.92(4.32-5.79)*10
/L, 14.8(13.217.08)g/dl
and 45.2(41.3-54.18)% for RBC, Hgb and Hct respectively for adult men and 4.39(3.565.16)*10
12
/L, 13(9.3-15.9)g/dl and 41(32.6-46.2)% for RBC, Hgb and Hct, respectively for pregnant
women. The median and 95% RI for WBC parameters and platelet count were 7.5(4.14-11.5)*10
2.2(1.2-5.21)*10
9
/L, 0.7(0.3-1.2)*10
9
/L, 4.8(2.06-7.88)*10
9
/L, and 227(152-353.3)*10
/L WBC count,
absolute lymphocyte, absolute mixed cell and absolute neutrophil and platelet count for adult men and
7.5(4.55-12.4)*10
9
/L, 2.2(1.2-3.9)*10
9
/L, 0.7(0.3-1.2)*10
9
/L, 4.9 (1.9-10.1)*10
/L and 202(142362)*10
9
/L WBC, Lymphocyte, Mixed cell, Granulocyte and platelet count for male and pregnant
women, respectively.
Conclusion:- The result from the current study identified that there is variation in most of the reference
interval of hematological parameters especially red cell parameters with reference intervals conducted in
other parts of the country, Africans as well as western countries.
Description
Keywords
Reference interval, Hematological Parameters, Arbaminch, Ethiopia