Establishment of hematological reference intervals for apparently healthy adult residents in Asella town, southeast Ethiopia
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Date
2019-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Abeba University
Abstract
Background: About 70% of medical decisions made by physicians are based on the information
presented by laboratory results. However, test results by themselves are valueless unless reported
with the appropriate reference interval or medical decision limit. Currently, Ethiopia use reference
intervals adopted from textbooks that refer mainly to Caucasian subjects. The country having
heterogeneous population, there is a need to establish locally derived hematological reference
interval that could be used in Asella town, Arsi zone, Ethiopia.
Objective: To establish hematological reference intervals for apparently healthy adults in Asella
town, southeast, Ethiopia.
Methods: A cross sectional study was conducted from January to March 2019 on apparently
healthy individuals in Asella town aged from 18-60 years. Predesigned and structured
questionnaire was used for collection of data on socio-demographic characteristics and dietary
pattern of the reference population. Systematic random sampling technique was used. About 3ml
of EDTA whole blood was collected and analyzed using Sysmex KX 21N automated hematology
analyzer which analyses 60 tests per hour. The data was entered and analyzed by appropriate
statistical software (Epi Info and SPSS) and interpreted using non-parametric methods, by which
central 95% of the measured values was included in the intervals.
Result: a total of 494 participants were recruited and 424 participants were involved in this study
with the median age of 28 years. Except WBC (3.4 to 10.1 x10
9
/L) which showed no significant
difference, other men and non-pregnant women reference intervals are: RBC (4.77 to 6.07 x10
/L;
4.18 to 5.29 x10
12
/L), HGB (14.7 to 18.1 gm/dL; 12.7 to 15.7gm/dL), HCT (42.1 to 51.3%; 37.1
to 44.4%) and PLT (159 to 336 x10
9
/L; 177 to 376 x10
9
/L), respectively. Pregnant women’s WBC,
RBC, HGB and PLT are (4.9 to 13.2 x10
9
/L, 3.58 to 4.9 x10
12
/L, 11.0 to 14.6gm/dL and (138 to
368 x10
9
/L), respectively.
Conclusion: most of the hematological RI of this study was significantly different from currently
in use in Asella referral and teaching hospital laboratory. The difference was also observed in
studies of other African countries as well as studies from different parts of Ethiopia.
12
Description
Keywords
Hematological RI, Asella, men, non-pregnant women, pregnant women.