College of Veterinary Medicine and Agriculture

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Browsing College of Veterinary Medicine and Agriculture by Author ". Dr.Fufa Abunna"

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    Assessment of Staphylococcus aureus and Methicillin-Resistant S. aureus (MRSA) in Dairy Farms of Sheger City, Oromia, Ethiopia: A One Health approach
    (Addis Abeba University, 2025) Makida Elias; . Dr.Fufa Abunna; Dr.Beteley Tekola
    Antimicrobial resistance (AMR), particularly involving S. aureus and methicillin-resistant S. aureus (MRSA), presents a growing public health concern in low- and middle-income countries such as Ethiopia. A cross-sectional study was carried out to investigate the occurrence, antimicrobial resistance profiles and environmental risk factors of S. aureus and MRSA in dairy farms in Sheger City, Oromia Region, using a One Health approach. A total of 312 sample comprising 212 udder milk, 40 hand swabs, and 60 environmental swab were collected from December 2024 to May 2025. Bacterial isolation was performed using conventional culture methods and confirmed by MALDI-TOF MS and antimicrobial susceptibility was tested using the disk diffusion method according to CLSI, (2022) guidelines. Out of the 312 samples, 65 (20.83%) were found to be positive for S. aureus, and 31 (9.9%) were identified as MRSA. The prevalence of S. aureus was 23.11% in udder milk, 27.5% in hand swabs, and 8.3% in environmental swabs. MRSA was detected only in udder milk samples, with a prevalence of 14.62%. All isolates exhibited multidrug resistance (MDR), with 100% resistance to amoxicillin, penicillin, tetracycline, clindamycin, and cloxacillin. High susceptibility was observed for ciprofloxacin, gentamicin, and norfloxacin according to Interpretive categories and zone diameter breakpoint (CLSI, 2022). Significant associations were found between S. aureus prevalence and poor hygiene practices, including inadequate handwashing, udder cleaning, and ubstandard drainage systems.The detection of S. aureus and MRSA particularly the exclusive occurrence of MRSA in milk samples raises serious concerns regarding milk safety and the potential for zoonotic transmission. The widespread multidrug resistance among isolates further emphasizes the need for targeted interventions. These findings call for urgent improvements in farm hygiene, promotion of prudent antimicrobial use and implementation of integrated AMR surveillance systems to mitigate the risks to animal and public health.