Production and Characterization of starch Based Antimicrobial Packaging Film through Incorporation of Plumbago Zeylanica (Amira) Root Crude Extract
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Date
2018-06
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Publisher
AAU
Abstract
Antimicrobial packaging is one of the most promising active packaging systems. Starch based
film is considered an economical and environmentally-friendly material for antimicrobial
packaging. This study aimed at the development of food packaging from corn starch
incorporated with methanol extract of plumbago zeylanica root as antimicrobial agents. The
antimicrobial activity of the extract was tested on three bacterial strains namely Staphylococcus
aureus, Salmonella typhi, E. coli and one fungal strain known as aspergilla niger. Inhibition of
bacterial growth was examined using zone of inhibition test on solid media. For this 200 µl of
various concentrations, 200mg/ml 100mg/ml, 50mg/ml and 25mg/ml plant extract were added
into 8 mm hole diameter using micropipette. Hence 200µl of 200mg/ml extract could inhibit
13.5, 8.75, 10.5 and 27 mm diameter for Salmonella typhi, E. coli, staphylococcus aureus and
Aspergilla niger respectively. Minimum inhibitory concentration was determined involving broth
dilution method. From minimum inhibitory concentration findings, 1.25mg/ml, 2.5mg/ml
,1.25mg/ml and 0.625 mg/ml crude extract in liquid medium completely inhibited the growth of
S. aureus, E. coli, Salmonella typhi and aspergilla niger respectively. This result showed that
plumbago zeylanica exhibited a potential antimicrobial activity on selected pathogenic
microorganisms. Consequently, the control and antimicrobial agent films were produced by
casting method. From the observations, antimicrobial packaging films exhibited inhibitory zones.
Interestingly, a wide clear zone on solid media was observed for Salmonella typhi and aspergilla
niger growth inhibition whereas inhibition for E. coli and Staphylococcus aureus were not as
effective as Salmonella typhi and aspergilla niger. These results showed that the incorporation of
plumbago zeylanica extract had a significant influence on the growth of each selected
microorganism (P<0.05). Overall, the current study manifested that plasticized corn starch can be
regarded as a promising biodegradable antimicrobial packaging film.
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Keywords
Antimicrobial activity, Corn Starch, Packaging Film, Plumbago Zeylanica, Production