EVALUATION OF POTENCY AND IMMUNOGENECITY OF INACTIVATED CELL CULTURE RABIES VACCINE, PREPARED AT NATIONAL VETERINARY INSTITUTE, ETHIOPIA
| dc.contributor.advisor | Dr. Bedaso Mammo, Dr. Berecha Bayissa | |
| dc.contributor.author | ENDALKACHEW, BESHAH | |
| dc.date.accessioned | 2018-10-24T07:59:38Z | |
| dc.date.accessioned | 2023-11-30T12:54:16Z | |
| dc.date.available | 2018-10-24T07:59:38Z | |
| dc.date.available | 2023-11-30T12:54:16Z | |
| dc.date.issued | 2018-06 | |
| dc.description | A thesis submitted to the College of Veterinary Medicine and Agriculture of Addis Ababa University in partial fulfillment of the requirements for the degree of Master of Veterinary Science in Veterinary Microbiology | en_US |
| dc.description.abstract | Rabies is the first prioritized zoonotic disease threatening public health in Ethiopia. Mass vaccination is the best existing strategy to control canine rabies. The objective of the present research was to evaluate potency and immunogenicity of Evlelyn-Rokitnicki- Abelseth (ERA) inactivated cell culture anti-rabies vaccine producing in National Veterinary Institute (NVI). The vaccine was produced as per the institute’s production standard operation procedure (SOP). Potency test of the vaccine conducted in Swiss Albino mice according to National Institute of Health, America (NIH) potency test procedure and Verorab rabies vaccine was used as reference vaccine. However, the immunological experiment undertaken in three to four months old puppies. Randomly assigned eight puppies to the vaccinated group were subcutaneously administered 1ml of NVI’s rabies vaccine while other eight were kept as control. Their antibody response was monitored on 0, 7, 14, 21, 30 and 60 days post vaccination. With regards to potency, the median effective dose (ED50) of the test vaccine was 1.47 while the reference vaccine was 1.3. Therefore, relative potency (RP) of the test vaccine found to be 1.45IU/ml in 1ml of single recommended dose. On the other hand, all dogs recruited for immunological experiment had low antibody titer and the titer of control dogs remained at lower level over experiment period. However, the mean antibody titer of vaccinated puppies statistically rose to 1.556IU/ml on 7th day of post vaccination. Similarly, the mean titer increased for the next consecutive two weeks. The antibody titer reached peak of 3.585IU/ml on 30th day of post vaccination. Despite of its reduction, it stayed at higher level until the end of experiment. In general, the test vaccine is potent enough to meet requirement of veterinary inactivated rabies vaccine and is effective and immunogenic for two months of post vaccination. However, immunological experiment in dogs needs to be undertaken for longer period and complimented with protective efficacy clinical trial. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/13131 | |
| dc.language.iso | en | en_US |
| dc.subject | Dogs | en_US |
| dc.subject | Immunogenicity | en_US |
| dc.subject | Mice | en_US |
| dc.subject | Rabies Vaccine | en_US |
| dc.title | EVALUATION OF POTENCY AND IMMUNOGENECITY OF INACTIVATED CELL CULTURE RABIES VACCINE, PREPARED AT NATIONAL VETERINARY INSTITUTE, ETHIOPIA | en_US |
| dc.type | Thesis | en_US |