Local Use and Molecular Genetic Diversity Studies of Anchote (Coccinia Abyssinica (Lam.) Cogn.) in West Wollega, Oromia Region of Ethiopia
No Thumbnail Available
Date
2019-09-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Anchote (Coccinia abyssinica) is a multi-purpose, endemic and valuable food crop of Ethiopia. Currently great emphasis is placed on consumption of food that will provides nutrients and helps in prevention of disease. In spite of this facts, sufficient studies have not been conducted on local use and molecular genetic diversity of anchote. The objective of this study was to assess information on the local use and conservation, and to analyze molecular genetic diversity of anchote in west Wollega Zone of Oromia Regional State, Ethiopia. Local use of anchote data were collected through questionnaires, face to face interview and direct observation of cultivated anchote on farm land. Anchote seeds were collected in the form of gift or purchase from farmers for molecular genetic diversity study. The genetic diversity within and among accessions of anchote collected from six districts of west Wollega was assessed by use of ISSR marker. The result of the study showed that most of the farmers commonly produced, used and had sufficient experience on cultivating, usage, conservation, and management of anchote. Cultivation and management processes were found to be an important factor affecting yield quality and quantity of anchote crop. Six categories of use (food, cash income, medicine, cultural, fodder, and ornament) were recorded. Anchote crop has strong link with socio-economic and cultural, and medicinal purposes than any other crop. Different parts of the crop used for various purpose and the root was most utilized for food, medicinal and cultural purposes. The mother root or “gubo” (in Afan Oromo) is utilized part for medicinal purpose. The amplification of genomic DNA with six ISSR marker yielded 39 scorable loci, of which 79.49% were found polymorphic. The number of observed alleles ranged from 1.3846 - 1.4615 with mean of 1.7949. The effective number of alleles (ne), Nei’s gene (h), and genetic diversity estimated by Shannon’s information index (I) were 1.4314, 0.2544 and 0.3865 respectively. The total genetic diversity, Ht (0.2544 ± 0.0354) and the average intrapopulation genetic diversity (Hs, 0.1701± 0.0196,) with high level of gene flow (Nm=1.0096) between populations, reflecting high gene differentiation (Gst=0.3312). The AMOVA showed that high value of genetic variation was found within populations (90%), whereas a low value of genetic variance (10%) was found among populations. Cluster analysis of anchote using the NJ, PCoA and UPGMA method showed in traceable patterns of geographic origin and occasional intermixing. This is because of high gene flow among the groups. The obtained information on genetic variation between the genotype and population can be used for future conservation and breeding program of the anchote crops.
Description
Keywords
Anchote, Local Use, Genetic Diversity, Issr, West Wollega