The Effect of Herbal Preparations on Staphylococcus Aureus and Strepthlococcus Agalactlae Isolated from Clinical Bovine Mastitis

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Date

2004-06

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Addis Ababa University Faculty Of Veterinary Medicine

Abstract

A study was conducted in Debrc-Zdt town to determine the incidence of bovine clinica l mastitis in a purposefully se lected two dairy Fanns and the efficacy of co nventio nal antimicrobial drugs and traditional herbs. from September '20Q3·March 2004. The objectives of the work were to assess the invitro effect of six herbal preparations; namely , Bntt.:eo am idysemrica, Combermw molle, C) 'pllOsremmu adenocale PersiCllria Jt!l1egalen.*1is. Plantago Itlll ceo/ata and ZeJmeria ~cabra an major isolates of clinical bovine mastitis. to compare their efficacy. with co nventional anllmicrobial agents that are cOlllmon ly lIsed for the treatment of bovine mastitis. and to 1I11,:~$ ligatc the effect of the herbs on the gro\\ til inhibiti on o f n:sis tam isolates. The herbs \\>I.!re c(llh.:ctcd from their natural habitat~ and processed and extracted \\ilh 80% methanol. Both absolute methanol and aqueou~ extracts of Combt!r1/II1/ II/ol/l! were assessed foe antimicrobial prop~ny \!llk samples from clinical C3ses were colll!ch.:d <ts~ plicall~ and causa! agcnts wac idcntilicd alier the severity of the Ji..;c<t::;cs in ~ach cem was cilh:gori7Cd into Grade I-Grade III h~ rollo\\ing :-tanJarJ laboralOrj proceJurcs and linallj scnslmit} test was conJucted on .Staphyloc:ocnn flureus (n= 17) and ~"'trep{()cor.:clIJ agahu .. 'Oae (11 ' 1-0. which were the predominant isnlatcs. lhe incidence rate \\as 12..t neo\\ clinical ma stitis cast.::; IOOcows-monlh and 6.7 new clinical maMitis cases/IOOco\\ s .. month at risk in Et\RO amI Fair-field clair) farms rcspccti\cly \\lIh an l1Vcrali mCldt:llcc 01"9 ne\\- climcal masti tis cases IOOCows-111omh at ri sk. Staplz.liocot'(:lIJ "pecic ... H2.3%) amI Sfl'cpfOCOeUf.) sPCClt!S (34.5%) \\(.'n: lht! miljor isol31CS from Gradl! I (X-Jl'o) and Gmllc II lI6%) clinical casc~ Singh: infccllon Llf 46.8% and mixed 37 5°,l,I and cont3minatt:d 9.4% infectio ns were re corded \\ iIh 6.3% negative culturl!s. Swphylucocclls ClUl'ell.'f was resi stant to OxytctnlCjCllilC (::!3.5%) and Penicillin G (64.7°!(j) and Stl'f!prD(,'uCf..'II~ llgalllC.'rillf! was re sistant to :--Jcomycin and St reptonl)cin (S5 .7% each) and Oxytt.!tracycline (100%). 8m'cae cmrit{Hf!ntric:a, Comberrllm mol/e, CyphoJ'lemma adenocflu/t! and Persicaria senigalzt1si~i \\cre eneclive against susceptible and resistant isolates and among those absolute methanol extract of COmhl!rlllm molle sho\\ed a better eITcc l on both test organisms. Plol1lago lanceo/aw and Zelmeria scabru were not showing visible inhibitory zone against test organisms. None or the herbal extract preparations showed visible inhibitory effcct on E,'icJil!richi(1 coli fhis sluuy indicated that mastitis is a great problem in the two dairy famls and resistant isolate s arc circula ti ng wiLhin fanns . For this herbal preparations might be cons id ered as an alternative option for the treatment o f resistant isolates of clinical bovine ma stiti s for the future.

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Keywords

Clinica l ma stiti s. herbal prepara tions. aCliologicai agents, SWphylocoCCliS alll't!IlS, SlreptococC ll s a8"lacli"e, se nsitivit y. effec t, conventional antimicrob ials and resi stant isolates.

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