Antimicrobial Activity of Solvent-Extracts of Cucumis Ficifolius and Zehneria Scabra on Some Test Microorganisms
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Date
2011-08
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Addis Ababa University
Abstract
The crude chloroform, ethyl acetate, acetone, methanol and ethanol extract of the leaves
of Cucumis ficifolius and Zehneria scabra were tested against in vitro antibacterial
activity of five human pathogenic bacteria using disc diffusion method and agar dilution
method for Minimum Inhibitory Concentration (MIC). Pseudomonas aeruginosa and
Shigella boydii were susceptible test organism for the ethyl acetate and acetone extracts
of the leaves of C. ficifolius with inhibition zones of 14±0.577mm, 12±0.577mm and an
MIC of 0.781mg/ml, 3.125mg/ml respectively, but Escherichia coli and Salmonella typhi
were the most resistant bacteria to all extracts of the leaves of this plant species with no
inhibition zones. Staphylococcus aureus and E. coli were found to be the most
susceptible bacteria for the ethyl acetate and acetone extracts of the leaves of Z. scabra
with inhibition zones of 22.6±0.33mm, 14±0.577mm and MIC of 0.781mg/ml,
1.56mg/ml respectively. However S. typhi was the most resistant to all extracts of the
leaves of this plant with no inhibition zone. The acetone extracts of C. ficifolius and the
ethyl acetate extracts of Z. scabra were most potent but the chloroform extracts of both
medicinal plants were ineffective against all bacterial and fungal test organisms. C.
ficifolius and Z. scabra didn’t show any inhibitory activity against Botrytis and Fusarium.
It can be concluded that C. ficifolius and Z. scabra had shown antibacterial activity
having potential for further study to serve as source of antibacterial agents.
Key words and phrases: Antibacterial activity, antifungal activity, minimum inhibitory
concentration, Cucumis ficifolius, Zehneria scabra.
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Antibacterial activity, antifungal activity, minimum inhibitory concentration, Cucumis ficifolius, Zehneria scabra