In Vitro Propagation of Pterolobium Stellatum from Shoot Tip Explants
| dc.contributor.advisor | Feyissa, Tileye (PhD) | |
| dc.contributor.author | Jemaneh, Kafane | |
| dc.date.accessioned | 2019-10-01T12:17:10Z | |
| dc.date.accessioned | 2023-11-09T04:20:54Z | |
| dc.date.available | 2019-10-01T12:17:10Z | |
| dc.date.available | 2023-11-09T04:20:54Z | |
| dc.date.issued | 2019-01-02 | |
| dc.description.abstract | Pterolobium stellatum is ever green perennial dicotyledonous woody shrubs commonly known as redwing. It belongs to Fabaceae family and distributed in tropical and subtropical climates of South Africa to Eastern Africa. It is used as traditional medicine against various diseases in some Africa countries and as source of tannins for leather industries to produce ink. This plant is highly destructed or action of destroying the plants locally and has prolonged seed dormancy and low seed germination rate. Therefore, the objective of this study is to develop micro propagation protocol for Pterolobium stellatum from shoot tip explants. The seeds of P. stellatum were de-coated and surface sterilized with 70% ethanol for 5 minutes followed by 30% ,15% and 5% Clorox for 25, 15 and 5 minutes respectively. The de-coated and sterilized seeds were cultured on Murashige and Skoog medium containing different concentrations of 6-Benzylaminopurine (0.0, 0.5, 1.0.1.5, 2.0, 2.5, 3.0 and 3.5 mg/l), Kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/l) alone and combination of 6-Benzyl aminopurine (0.5- 3.0 mg/l) with α- Naphthalene Acetic acid (0.1, 0.25, 0.5 mg/l) on MS medium for shoot tip initiation and multiplication. Rooting was achieved by using half strength MS medium containing different concentrations of Indol-3-Butryric acid (0.0, 0.5, 1.5, 1.0, 2.0 mg/l) and α- Naphthalene Acetic acid (0.5, 1.0, 1.5, 2.0 mg/l). The highest percentage of shoot initiation (86.7%) and maximum numbers of shoots per explants (3.76±0.32) were obtained on MS medium fortified with 2.0 mg/l BAP alone. The highest shoot length was obtained on MS medium containing 1.5 mg/l KN combined with 0.1 mg/l NAA (4.45±.20 cm). The highest mean numbers of roots per explants was (1.93±0.21) and mean root length (1.73±0.17 cm) was obtained on MS medium containing 1.0 mg/l NAA alone. Among acclimatized plantlets, 43% survived in greenhouse. This protocol can be used as a starting protocol for further optimization for production of large number of planting materials. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/19267 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Micro Propagation | en_US |
| dc.subject | Shoot Multiplication | en_US |
| dc.subject | Rooting | en_US |
| dc.subject | Shoots Tips | en_US |
| dc.subject | Pterolobium Stellatum | en_US |
| dc.title | In Vitro Propagation of Pterolobium Stellatum from Shoot Tip Explants | en_US |
| dc.type | Thesis | en_US |