Micropropagation of Oreosyce Africana From Shoot Tips
| dc.contributor.advisor | Feyissa, Tileye (PhD) | |
| dc.contributor.author | Feyisa, Abdisa | |
| dc.date.accessioned | 2019-03-29T11:32:22Z | |
| dc.date.accessioned | 2023-11-09T04:20:44Z | |
| dc.date.available | 2019-03-29T11:32:22Z | |
| dc.date.available | 2023-11-09T04:20:44Z | |
| dc.date.issued | 2018-08-01 | |
| dc.description.abstract | Oreosyce africana (‘manabasi’ in Afan Oromo) is the formulated medicinal plant that kills Anopheles arabienses at both larvae and adult stage. The availability of this plant species is very limited and scarcely distributed in Ethiopia. Hence, this study is aimed to develop efficientmicro propagation protocol for this plant speciesusing shoot tip explants. Shoot tips were excised from mother plant and sterilized with 70% alcoholfor fifteen seconds followed by20% sodium hypochlorite (NaOHCl for 20 min and rinsed three times in sterile distilled water. The sterilized shoot tips were cultured on Murashige and Skoog (1962) (MS) medium supplemented with 0.05mg/l BAP. The initiated shoot tipswere transferred to MS medium supplemented with different concentrations of BAP alone, KIN alone, BAP in combination with IBA and KIN in combination with IBA for shoot multiplication. For rooting, half strength MS medium supplemented with various concentrations of IAA alone, IBA alone and IAA in combinations with IBAwere used. MS medium without PGRs was used as control. The result showed that highest mean shoot number (36.66±2.03) per explants was obtained on a medium supplemented with 0.5 mg/l BAP and the highest mean shoot height (4.26±0.10 cm) was obtained on growth regulators free medium.The highest mean root number (7.77±.44) and mean root length (5.42±.45 cm) were obtained on 1/2 strength MS medium containing 0.5mg/l IAA. Up on acclimatization, 98% plants survived. The results showed that, this micro propagation protocol can be used for mass propagation and ultimate conservation of this medicinal plant. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/17356 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Oreosyce Africana | en_US |
| dc.subject | Micro Propagation | en_US |
| dc.subject | Medicinal Plant | en_US |
| dc.subject | Larvicidal | en_US |
| dc.subject | Adulticidal | en_US |
| dc.subject | Plant Growth Regulator | en_US |
| dc.title | Micropropagation of Oreosyce Africana From Shoot Tips | en_US |
| dc.type | Thesis | en_US |