Evaluation and in Vitro Antimicrobial Effect Assessment of Ethyl Pyruvate's Efficacy Against Major Pathogens Associated with Bovine Mastitis
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Date
2025
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Addis Abeba University
Abstract
The treatment of bovine mastitis is complex due to its varied etiology and the growing challenge of antimicrobial resistance, which limits the efficacy of conventional antibiotic therapies, particularly in persistent infections or those caused by certain pathogens like Staphylococcus aureus. Ongoing research explores alternative treatments such as plantbased therapies. However, the efficacy and practical application of these alternatives are still under investigation. Cross sectional study was conducted in Bishoftu, Ethiopia, from November 2024 to May 2025. This study aimed to evaluate the antimicrobial effects of ethyl pyruvate (EP) on major pathogens of bovine mastitis and assess them in vitro susceptibility profiles. Bacteria were isolated and identified using selective media and MALDI-TOF MS from 90 milk samples of cows confirmed to have mastitis using CMT and SCC which resulted 59.3% were "strongly positive," 30.5% "distinct positive," and 10.2% "weakly positive," with a statistically significant correlation (p<0.01; r=0.653) with SCC result. Culturing revealed that 65.5% of the samples contained primarily Staphylococcus aureus (28.8%), Escherichia coli (21.1%) and streptococcus agalactiae 6.7%. Sensitivity tests were carried out with different concentration (100%, 75%, 50% and 25%) of ethyl pyruvate with conventional antibiotic disks to see resistance pattern. The antibacterial effects of EP were found to be concentration-dependent. EP75 was the most effective at a 29.82 mm zone of inhibition, while EP25 had the lowest efficacy at 16.89mm. A significant difference was found in susceptibility among bacterial species, with E. coli showing the highest susceptibility at 25.63± 0.53 mm and S. aureus the lowest at 17.65mm. Furthermore, 100% multi-drug-resistant to Tetracycline, Ampicillin, and Amoxicillin showed across all tested species. In vitro analyses indicate EP possesses a significant antimicrobial property against major pathogens of bovine mastitis. Further in vivo investigation is warranted to ascertain its therapeutic efficacy and comprehensive doseresponse studies to establish the optimal dosage regimens and administration routes for ethyl pyruvate practical application in field settings
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Keywords
: Bacteria, Bovine mastitis, Concentration, Ethyl pyruvate, susceptibility